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. 1979 Dec;76(12):6250-4.
doi: 10.1073/pnas.76.12.6250.

Cloning and expression of the Escherichia coli replication origin in a single-stranded DNA phage

J Kaguni, L S LaVerne, D S Ray

Cloning and expression of the Escherichia coli replication origin in a single-stranded DNA phage

J Kaguni et al. Proc Natl Acad Sci U S A. 1979 Dec.

Abstract

The Escherichia coli DNA replication origin (oriC) and the adjacent asparagine synthetase gene (asnA) have been inserted into the duplex replicative form DNA of the single-stranded phage vector M13Goril. By in vitro recombination, the entire oriC asnA-containing plasmid pJS5 was inserted into M13Gori1 in both possible orientations. Both phage types transduce the asnA gene and confer upon the M13 vector the ability to replicate as a plasmid in the E. coli mutant rep3. In rep+ hosts, these phages undergo single-stranded DNA synthesis and viral morphogenesis.

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References

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