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. 1985 Apr;16(3-4):99-101.
doi: 10.1007/BF01983111.

A sum of 14C-putrescine metabolites as a measure of DAO activity. Column chromatography assay

W A Fogel, M Ulatowska, K Adach, Z Osińska

A sum of 14C-putrescine metabolites as a measure of DAO activity. Column chromatography assay

W A Fogel et al. Agents Actions. 1985 Apr.

Abstract

The rapid ion-exchange chromatographic method was developed for the separation of 14C-putrescine from its metabolites arising from diamine oxidase (DAO, E.C. 1.4.3.6) catalysed reaction. The assay is linear for putrescine conversion with both, time and enzyme concentration. With this method many samples could be analysed simultaneously.

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