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. 2024 Sep 26;391(12):1157-1159.
doi: 10.1056/NEJMc2405937. Epub 2024 Sep 11.

Sequencing-Based Detection of Avian Influenza A(H5N1) Virus in Wastewater in Ten Cities

Michael J Tisza  1 , Blake M Hanson  2 , Justin R Clark  1 , Li Wang  1 , Katelyn Payne  1 , Matthew C Ross  1 , Kristina D Mena  2 , Anna Gitter  2 , Sara J Javornik Cregeen  1 , Juwan Cormier  1 , Vasanthi Avadhanula  1 , Austen Terwilliger  1 , John Balliew  3 , Fuqing Wu  2 , Janelle Rios  2 , Jennifer Deegan  2 , Pedro A Piedra  4 , Joseph F Petrosino  4 , Eric Boerwinkle  5 , Anthony W Maresso  6
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Sequencing-Based Detection of Avian Influenza A(H5N1) Virus in Wastewater in Ten Cities

Michael J Tisza et al. N Engl J Med. .
No abstract available

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Figures

Figure 1:
Figure 1:. Influenza A H5N1 serotype is detectable in wastewater via virome sequencing.
(A) Dereplicated reads from wastewater virome sequencing assigned to H5N1 from all samples with validated H5N1 signal (see methods) aligned to a recently collected H5N1 2.3.4.4b reference sequence (GISAID: EPI_ISL_19027114). (B) Temporal chart (September 1st, 2023 – July 15th, 2024) measuring Influenza A serotype and abundance averaged across all 23 Texas wastewater treatment sites (colored bars) and hospital inpatient rates due to influenza across Texas as reported by Texas Department of Health and Human services (black line). Note that inpatient rates also include those caused by Influenza B, which is not measured in wastewater in this chart. (C) Bars represent proportion of influenza A serotypes in wastewater samples from each city. Grey line shows the 3-week moving average of influenza A relative abundance in wastewater, normalized to each city’s seasonal maximum. Note that sampling of South Texas City I sites began April 8th, 2024.
See this image and copyright information in PMC

References

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    1. H5N1 Bird Flu: Current Situation Summary. 2024. (Accessed July 27th, 2024, at https://www.cdc.gov/flu/avianflu/avian-flu-summary.htm.)
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    1. Clark JR, Terwilliger A, Avadhanula V, et al. Wastewater pandemic preparedness: Toward an end-to-end pathogen monitoring program. Front Public Health 2023;11:1137881. - PMC - PubMed
    1. Tisza M, Javornik Cregeen S, Avadhanula V, et al. Wastewater sequencing reveals community and variant dynamics of the collective human virome. Nat Commun 2023;14:6878. - PMC - PubMed

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