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. 2024;39(3):ME24028.
doi: 10.1264/jsme2.ME24028.

Ferrihydrite Addition Activated Geobacteraceae, the Most Abundant Iron-reducing Diazotrophs, and Suppressed Methanogenesis by Heterogeneous Methanogens in Xylan-amended Paddy Soil Microcosms

Yoko Masuda  1   2 , Mitsutaka Chihara  1 , Keishi Senoo  1   2
Affiliations

Ferrihydrite Addition Activated Geobacteraceae, the Most Abundant Iron-reducing Diazotrophs, and Suppressed Methanogenesis by Heterogeneous Methanogens in Xylan-amended Paddy Soil Microcosms

Yoko Masuda et al. Microbes Environ. 2024.

Abstract

Paddy fields are a major emission source of the greenhouse gas methane. In the present study, the addition of ferrihydrite to xylan-amended paddy soil microcosms suppressed methane emissions. PCR-based and metatranscriptomic ana-lyses revealed that the addition of ferrihydrite suppressed methanogenesis by heterogeneous methanogens and simultaneously activated Geobacteraceae, the most abundant iron-reducing diazotrophs. Geobacteraceae may preferentially metabolize xylan and/or xylan-derived carbon compounds that are utilized by methanogens. Geomonas terrae R111 utilized xylan as a growth substrate under liquid culture conditions. This may constitute a novel mechanism for the mitigation of methane emissions previously observed in ferric iron oxide-applied paddy field soils.

Keywords: ferrihydrite; iron-reducing diazotrophs; metatranscriptome; methanogenesis; paddy field soil.

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Figures

Fig. 1.
Fig. 1.
Methane accumulation in vials from control (N), ferrihydrite-amended (NF), xylan-amended (X), xylan and ferrihydrite-amended (XF), cellulose-amended (C), and cellulose and ferrihydrite-amended (CF) paddy soils (mean±SD). * indicates ferrihydrite-amended soils, in which the accumulation of methane was significantly lower than in the sample without ferrihydrite (the Mann–Whitney U test; P<0.05).
Fig. 2.
Fig. 2.
Ratio of the number of mcrA transcript reads to the number of rRNA transcript reads (A) and the taxonomic composition of mcrA-derived archaea (B) obtained by a metatranscriptomic ana­lysis of xylan-amended (X) and xylan and ferrihydrite-amended (XF) paddy soils on Day 16 (mean±SD). * indicates ferrihydrite-amended soils, in which the mcrA ratio was significantly lower than in soils without ferrihydrite (the Mann–Whitney U test; P<0.05).
Fig. 3.
Fig. 3.
Ratio of the number of nifD transcript reads to the number of rRNA transcript reads (A) and the taxonomic composition of nifD-transcribing microbes (B) obtained by a metatranscriptomic ana­lysis of xylan-amended (X) and xylan and ferrihydrite-amended (XF) paddy soils on Day 16 (mean±SD). * indicates ferrihydrite-amended soils, in which the nifD ratio was significantly higher than in soils without ferrihydrite (the Mann–Whitney U test; P<0.05).
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