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. 1985 Apr;6(1-2):5-14.
doi: 10.1016/0143-4160(85)90030-2.

Calcium clamp of the intracellular environment

P F Baker, D E Knight, J A Umbach

Calcium clamp of the intracellular environment

P F Baker et al. Cell Calcium. 1985 Apr.

Abstract

Quantitative analysis of the effects of calcium on cell function requires methods for altering intracellular free Ca in a precise and reproducible manner. Microinjection of Ca is very unreliable largely because of the powerful Ca-binding properties of cytoplasm. Much more satisfactory are microinjection of Ca-buffers - provided enough buffer is introduced - and various forms of intracellular dialysis and perfusion which permit full equilibration of the cell interior with a defined artificial intracellular environment.

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