Identification of a novel force-generating protein, kinesin, involved in microtubule-based motility
- PMID: 3926325
- PMCID: PMC2851632
- DOI: 10.1016/s0092-8674(85)80099-4
Identification of a novel force-generating protein, kinesin, involved in microtubule-based motility
Abstract
Axoplasm from the squid giant axon contains a soluble protein translocator that induces movement of microtubules on glass, latex beads on microtubules, and axoplasmic organelles on microtubules. We now report the partial purification of a protein from squid giant axons and optic lobes that induces these microtubule-based movements and show that there is a homologous protein in bovine brain. The purification of the translocator protein depended primarily on its unusual property of forming a high affinity complex with microtubules in the presence of a nonhydrolyzable ATP analog, adenylyl imidodiphosphate. The protein, once released from microtubules with ATP, migrates on gel filtration columns with an apparent molecular weight of 600 kilodaltons and contains 110-120 and 60-70 kilodalton polypeptides. This protein is distinct in molecular weight and enzymatic behavior from myosin or dynein, which suggests that it belongs to a novel class of force-generating molecules, for which we propose the name kinesin.
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References
-
- Adams RJ, Bray D. Rapid transport of foreign particles microinjected into crab axons. Nature. 1983;303:718–720. - PubMed
-
- Allen RD, Metuzals J, Tasaki I, Brady ST, Gilbert SP. Fast axonal transport in squid giant axon. Science. 1982;218:1127–1128. - PubMed
-
- Asai DJ, Wilson L. A latent activity dynein-like cytoplasmic magnesium adenosine triphosphatase. J Biol Chem. 1985;260:699–702. - PubMed
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