Chlordiazepoxide-induced hyperphagia in non-food-deprived rats: effects of Ro15-1788, CGS 8216 and ZK 93 426

Abstract

Chlordiazepoxide (1.25-20.0 mg/kg i.p.) was administered to non-food-deprived male rats given 30 min access to a highly palatable, familiar diet, and produced a potent stimulation of food consumption. At the maximum dose effect, the rats consumed about 24 g food in the 30 min test. The benzodiazepine receptor antagonist, Ro15-1788 (2.5-40.0 mg/kg i.p.) had no effect on food intake when given alone, but did dose-dependently attenuate chlordiazepoxide's hyperphagic effect. The antagonist CGS 8216 (5.0-20.0 mg/kg i.p.) completely abolished the hyperphagic effect, and in doses of 10.0 and 20.0 mg/kg produced significant suppression of feeding when administered by itself. ZK 93 426, in doses (0.625-10.0 mg/kg i.p.) which have previously been shown to antagonize the discriminative cue of chlordiazepoxide produced no significant change in chlordiazepoxide's hyperphagic effect. These data point to an interesting and important distinction between ZK 93426 and the other two benzodiazepine receptor antagonists when given in combination with chlordiazepoxide in the palatable food consumption test.