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. 1985 Sep;33(9):965-8.
doi: 10.1177/33.9.3926869.

A fluorescence staining method for the demonstration and measurement of lysosomal enzyme activities in single cells

G P Luyten, A T Hoogeveen, H Galjaard

A fluorescence staining method for the demonstration and measurement of lysosomal enzyme activities in single cells

G P Luyten et al. J Histochem Cytochem. 1985 Sep.

Abstract

A cytochemical fluorescence method is described that makes possible simple, rapid, and specific demonstration and measurement of the activities of a wide variety of lysosomal enzymes in single cells using 4-methylumbelliferyl derivatives as substrates. The validity of the method and a number of applications using normal and mutant human cells are presented.

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