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. 2024 Aug 23;10(17):e36690.
doi: 10.1016/j.heliyon.2024.e36690. eCollection 2024 Sep 15.

Assessment of the presence of multidrug-resistant Escherichia coli, Salmonella and Staphylococcus in chicken meat, eggs and faeces in Mymensingh division of Bangladesh

Kazi Rafiq  1 , Aminatu Abubakar Sani  1   2 , Muhammad Tofazzal Hossain  3 , Md Tarek Hossain  1 , Md Hadiuzzaman  3 , Mohammad Abdus Sattar Bhuiyan  1   4
Affiliations

Assessment of the presence of multidrug-resistant Escherichia coli, Salmonella and Staphylococcus in chicken meat, eggs and faeces in Mymensingh division of Bangladesh

Kazi Rafiq et al. Heliyon. .

Abstract

The emergence of bacteria that is resistant to several drugs of clinical importance poses a threat to successful treatment, a phenomenon known as multidrug resistance that affects diverse classes of antibiotics. The purpose of this study was to evaluate the prevalence of multidrug-resistant Escherichia coli, Salmonella spp. and Staphylococcus aureus in chicken egg, meat and faeces from four districts of Bangladesh. A total of 120 chicken samples were collected from different poultry farms. Conventional culture and molecular detection methods were used for identification of bacterial isolates from the collected samples followed by antibiotic susceptibility test through the disc diffusion method, finally antibiotic resistant genes were detected by PCR. E. coli, Salmonella spp. and Staphylococcus aureus were detected in meat, egg and faecal samples. Antimicrobial susceptibility results revealed isolates from faeces were 100 % resistant to amoxicillin, while all S. aureus and Salmonella sp. from faeces were resistant to doxycycline, tetracycline and erythromycin. Salmonella spp. isolates from eggs indicated 100 % resistance to erythromycin, amoxycillin, while E. coli were 100 % resistant to erythromycin. E. coli and S. aureus from meat were 100 % resistant to amoxicillin and erythromycin. However, Salmonella spp. from eggs were 100 % susceptible to doxycycline, gentamicin, levofloxacin and tetracycline. The mecA and aac(3)-IV genes were only found in S. aureus and E. coli, respectively. The Sul1, tetB, and aadA1 were highest in Salmonella spp. and S. aureus, while the sul1, tetA and bla SHV were higher in E. coli. Isolates from all samples were multidrug resistant. These findings indicate a high risk of transmission of resistance genes from microbial contamination to food of animal origin. The study emphasizes the need for effective biosecurity measures, responsible antibiotic use, and strict regulations in poultry production to prevent the spread of antibiotic resistance.

Keywords: AMR; Bangladesh; Foodborne pathogens; MDR bacteria; Poultry.

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Conflict of interest statement

The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Kazi Rafiq reports financial support, administrative support, and equipment, drugs, or supplies were provided by 10.13039/100020982Bangladesh Agricultural Research Council, Dhaka, Bangladesh. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Fig. 1
Fig. 1
Antimicrobial resistance pattern of E. coli,Salmonella spp. and S.aureus isolated from poultry faeces in Mymensingh division. Infectious agents (bacteria) are classified as "susceptible as S," "intermediate as I," or "resistant as R″ to specific antibiotics.
Fig. 2
Fig. 2
Antimicrobial resistance pattern of E. coli and S.aureus isolated from poultry meat in Mymensingh division. Infectious agents (bacteria) are classified as "susceptible as S," "intermediate as I," or "resistant as R″ to specific antibiotics.
Fig. 3
Fig. 3
Resistance pattern of E. coli,Salmonella spp. and S.aureus isolated from poultry eggs in Mymensingh division. Infectious agents (bacteria) are classified as "susceptible as S," "intermediate as I," or "resistant as R″ to specific antibiotics.
Fig. 4
Fig. 4
Genotypic resistance pattern in E.coli.
Fig. 5
Fig. 5
Genotypic resistance pattern in Salmonella spp.
Fig. 6
Fig. 6
Genotypic resistance pattern in Staphylococcusaureus.
See this image and copyright information in PMC

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References

    1. Tiseo K., Huber L., Gilbert M., Robinson T.P., Van Boeckel T.P. Global trends in antimicrobial use in food animals from 2017 to 2030. Antibiotics. 2020;9(12):918. - PMC - PubMed
    1. Aworh M.K., Kwaga J., Okolocha E., Mba N., Thakur S. Prevalence and risk factors for multi-drug resistant Escherichia coli among poultry workers in the Federal Capital Territory, Abuja, Nigeria. PLoS One. 2019;14(11) - PMC - PubMed
    1. Ferdous M.R., Ahmed M.R., Khan S.H., Mukta M.A., Anika T.T., Hossain M.T., Islam M.Z., Rafiq K. Effect of discriminate and indiscriminate use of oxytetracycline on residual status in broiler soft tissues. Vet. World. 2020;13(1):61. - PMC - PubMed
    1. Nisha A.R. Antibiotic residues - a global health Hazard. Vet. World. 2008;1:375–377.
    1. Singh S., Shukla S., Tandia N., Kumar N., Paliwal R. Antibiotic residues: a global challenge. Pharma Sci. Monit. 2014;5:184–197.

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