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. 2024 Oct 16;62(10):e0097524.
doi: 10.1128/jcm.00975-24. Epub 2024 Sep 16.

Specimen adequacy assay controls in nucleic acid amplification tests do not correlate with nasopharyngeal swab collection method

Katharine H D Crawford  1 , Mary Lynn Baniecki  2 , Elizabeth G Dushin  3 , Cassandra A Tierney  3 , Shunjie Guan  2 , Laurence L Stensland  1 , Ailyn C Perez-Osorio  1 , Alexander L Greninger  1   4
Affiliations

Specimen adequacy assay controls in nucleic acid amplification tests do not correlate with nasopharyngeal swab collection method

Katharine H D Crawford et al. J Clin Microbiol. .
No abstract available

Keywords: control; housekeeping gene; internal control; nasopharyngeal swab; specimen adequacy; swab.

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Conflict of interest statement

A.L.G. reports contract testing from Abbott, Cepheid, Novavax, Pfizer, Janssen, and Hologic, as well as research support from Gilead. M.L.B., E.G.D., C.A.T., and S.G. are employees of Pfizer, Inc.

Figures

Fig 1
Fig 1
Cycle threshold (Ct) values for the RPP30 and β-globin qPCR assays. “Good” nasopharyngeal swabs were collected from the nasopharynx with rotation for at least 5 seconds per naris, while “suboptimal” swabs were similarly inserted but for only one rotation of the swab (<3 seconds). “Poor” quality swabs did not fully reach the nasopharyngeal cavity and included no swab rotation. Significant differences in Ct value are shown on the plot (ANOVA test, *P < 0.05 and **P < 0.01). The horizontal black line indicates median Ct values. The samples whose RPP30 or β-globin genes did not amplify (see Table 1) are not included in this analysis. Suboptimal is abbreviated to “subopt.” in the x-axis labels for space considerations.
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