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. 2024 Aug 30:19:100886.
doi: 10.1016/j.onehlt.2024.100886. eCollection 2024 Dec.

Broad range molecular detection methods identify only Borrelia spp. in erythema migrans biopsies and blood of tick-bitten patients

Philippe Pérot  1   2 , Laura Tondeur  3 , Sara Moutailler  4 , Delphine Chrétien  1   2 , Nicole Corre-Catelin  5 , Muriel Vayssier-Taussat  6 , Marc Eloit  1   2   7 , Catherine Chirouze  8   9 , Céline Cazorla  10 ; OHTICKS Consortium
Collaborators, Affiliations

Broad range molecular detection methods identify only Borrelia spp. in erythema migrans biopsies and blood of tick-bitten patients

Philippe Pérot et al. One Health. .

Abstract

In this multicenter study conducted in France, we challenged the hypothesis of the transmission of pathogens other than Borrelia spp. in 22 patients developing erythema migrans following a tick bite. Using a combination of high-throughput microfluidic PCRs and agnostic metagenomics on skin biopsies and blood samples, no microorganisms other than Borrelia spp. was found.

Keywords: Erythema migrans; Lyme disease; Metagenomics; Pathogen discovery; RT-PCR; Ticks.

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Conflict of interest statement

The authors declare they have no conflicts of interest.

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