Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Sep 17;51(1):990.
doi: 10.1007/s11033-024-09905-8.

Exploring the neuroprotective potential of KC14 peptide from Cyprinus carpio against oxidative stress-induced neurodegeneration by regulating antioxidant mechanism

Madhumitha Vijayanand  1 , Praveen Kumar Issac  2 , Manikandan Velayutham  1 , Mohammed Rafi Shaik  3 , Shaik Althaf Hussain  4 , Ajay Guru  5
Affiliations

Exploring the neuroprotective potential of KC14 peptide from Cyprinus carpio against oxidative stress-induced neurodegeneration by regulating antioxidant mechanism

Madhumitha Vijayanand et al. Mol Biol Rep. .

Abstract

Background: Oxidative stress, a condition characterized by excessive production of reactive oxygen species (ROS), can cause significant damage to cellular macromolecules, leading to neurodegeneration. This underscores the need for effective antioxidant therapies that can mitigate oxidative stress and its associated neurodegenerative effects. KC14 peptide derived from liver-expressed antimicrobial peptide-2 A (LEAP 2 A) from Cyprinus carpio L. has been identified as a potential therapeutic agent. This study focuses on the antioxidant and neuroprotective properties of the KC14 peptide is to evaluate its effectiveness against oxidative stress and neurodegeneration.

Methods: The antioxidant capabilities of KC14 were initially assessed through in silico docking studies, which predicted its potential to interact with oxidative stress-related targets. Subsequently, the peptide was tested at concentrations ranging from 5 to 45 µM in both in vitro and in vivo experiments. In vivo studies involved treating H2O2-induced zebrafish larvae with KC14 peptide to analyze its effects on oxidative stress and neuroprotection.

Results: KC14 peptide showed a protective effect against the developmental malformations caused by H2O2 stress, restored antioxidant enzyme activity, reduced neuronal damage, and lowered lipid peroxidation and nitric oxide levels in H2O2-induced larvae. It enhanced acetylcholinesterase activity and significantly reduced intracellular ROS levels (p < 0.05) dose-dependently. Gene expression studies showed up-regulation of antioxidant genes with KC14 treatment under H2O2 stress.

Conclusions: This study highlights the potent antioxidant activity of KC14 and its ability to confer neuroprotection against oxidative stress can provide a novel therapeutic agent for combating neurodegenerative diseases induced by oxidative stress.

Keywords: Antioxidant; KC14; Neuroprotective; Oxidative stress; Peptide; Zebrafish models.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Teleanu DM, Niculescu A-G, Lungu II et al (2022) An overview of oxidative stress, Neuroinflammation, and neurodegenerative diseases. Int J Mol Sci 23:5938. https://doi.org/10.3390/ijms23115938 - DOI
    1. Liu Z, Zhou T, Ziegler AC et al (2017) Oxidative stress in neurodegenerative diseases: from Molecular mechanisms to clinical applications. Oxid Med Cell Longev 2017:1–11. https://doi.org/10.1155/2017/2525967 - DOI
    1. Koutsilieri E, Scheller C, Tribl F, Riederer P (2002) Degeneration of neuronal cells due to oxidative stress—microglial contribution. Parkinsonism Relat Disord 8:401–406. https://doi.org/10.1016/S1353-8020(02)00021-4 - DOI
    1. Kim GH, Kim JE, Rhie SJ, Yoon S (2015) The role of oxidative stress in neurodegenerative diseases. Exp Neurobiol 24:325–340. https://doi.org/10.5607/en.2015.24.4.325 - DOI
    1. Sayre LM, Perry G, Smith MA (2008) Oxidative stress and neurotoxicity. Chem Res Toxicol 21:172–188. https://doi.org/10.1021/tx700210j - DOI

MeSH terms

LinkOut - more resources