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. 2024 Sep 3:12:1445928.
doi: 10.3389/fcell.2024.1445928. eCollection 2024.

Effects of platelet-rich fibrin on human endometrial stromal cells behavior in comparison to platelet-rich plasma

Guanghui Yuan  1   2   3 , Duan Li  1   2   3 , Xin Du  1   2   3 , Yingxue Liu  1   2   3 , Xiaoxiao Wang  1   2   3 , Cuifang Hao  1   2   3
Affiliations

Effects of platelet-rich fibrin on human endometrial stromal cells behavior in comparison to platelet-rich plasma

Guanghui Yuan et al. Front Cell Dev Biol. .

Abstract

Introduction: Intrauterine transfusion of platelet-rich plasma (PRP) has become a new treatment for thin endometrium (TE) in recent years, but its low efficacy due to rapid release of growth factors limits its clinical use. Platelet-rich fibrin (PRF) starts the coagulation cascade reaction immediately after the blood comes into contact with the test tube. The natural coagulation process results in stable platelet activation and the slow release of growth factors.

Methods: In our study, primary human endometrial stromal cells (hESCs) were extracted from endometrial tissue. PRP and PRF were prepared from the patient cubital vein blood. Stromal cells were cultured in conditioned medium supplemented with PRP and PRF. Differences in cell behavior were observed by cell proliferation test and cell migration test. The relative expression levels of apoptotic Bax and antiapoptotic Bcl-2 genes were measured by qRT-PCR. The release of growth factors from PRP and PRF was detected by ELISA.

Results: We found that both PRP and PRF inhibited apoptosis of hESCs, which favored cell proliferation and migration. In addition, PRF releases growth factors for a longer period of time compared to PRP.

Discussion: PRF offer a more sustained therapeutic effect compared to PRP, which provides a new idea for endometrial regeneration and repair.

Keywords: cell apoptotic; endometrial stromal cells; platelet-rich fibrin; platelet-rich plasma; thin endometrium.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

FIGURE 1
FIGURE 1
Characteristics of PRF. (A) PRF preparation process. (B) Structure of PRF under scanning electron microscope. (a) Image of the PRF surface. (b) Image of the PRF cross-section. Scale bars = 10 μm.
FIGURE 2
FIGURE 2
Characterization of hESCs. (A) Morphology of cultured primary cells at different time point. (B) Immunofluorescent imaging data was presented as vimentin expression after culture.
FIGURE 3
FIGURE 3
Effects of PRP and PRF on the proliferation of hESCs at 24, 48, 72 and 96 h ***P < 0.001 compared with control group; **P < 0.01 compared with control group; *P < 0.05 compared with control group.
FIGURE 4
FIGURE 4
Effect of PRP and PRF on the migration of hESCs. (A) Normalized cell migration demonstrated a significant increase in 1dPRP and 14dPRF groups. (B) Cell migration was assessed after 24 h. Scale bars = 100 μm ***P < 0.001 compared with control group; **P < 0.01 compared with control group; ns, no significance.
FIGURE 5
FIGURE 5
Effects of PRP and PRF on the gene expression in hESCs. (A) Apoptosis gene Bax expression. (B) Anti-apoptosis gene Bcl-2 expression. ***P < 0.001 compared with control group; **P < 0.01 compared with control group; ns: no significance.
FIGURE 6
FIGURE 6
Growth factor release curve. (A) Total amount of VEGF released at different time points. (B) Total amount of TGF- β1 released at different time points. (C) Total amount of PDGF-AB released at different time points.
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