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. 2024 Sep 18;40(10):323.
doi: 10.1007/s11274-024-04134-4.

The divergence of DHN-derived melanin pathways in Metarhizium robertsii

Linan Xie #  1 , Yang Liu #  1 , Yujie Zhang #  2 , Kang Chen  1 , Qun Yue  1 , Chen Wang  1 , Baoqing Dun  2 , Yuquan Xu  1 , Liwen Zhang  3
Affiliations

The divergence of DHN-derived melanin pathways in Metarhizium robertsii

Linan Xie et al. World J Microbiol Biotechnol. .

Abstract

The important role of dihydroxynaphthalene-(DHN) melanin in enhancing fungal stress resistance and its importance in fungal development and pathogenicity are well-established. This melanin also aids biocontrol fungi in surviving in the environment and effectively infecting insects. However, the biosynthetic origin of melanin in the biocontrol agents, Metarhizium spp., has remained elusive due to the complexity resulting from the divergence of two DHN-like biosynthetic pathways. Through the heterologous expression of biosynthetic enzymes from these two pathways in baker's yeast Saccharomyces cerevisiae, we have confirmed the presence of DHN biosynthesis in M. roberstii, and discovered a novel naphthopyrone intermediate, 8, that can produce a different type of pigment. These two pigment biosynthetic pathways differ in terms of polyketide intermediate structures and subsequent modification steps. Stress resistance studies using recombinant yeast cells have demonstrated that both DHN and its intermediates confer resistance against UV light prior to polymerization; a similar result was observed for its naphthopyrone counterpart. This study contributes to the understanding of the intricate and diverse biosynthetic mechanisms of fungal melanin and has the potential to enhance the application efficiency of biocontrol fungi such as Metarhizium spp. in agriculture.

Keywords: Metarhizium spp.; Dihydroxynaphthalene-(DHN) melanin; Entomopathogenic fungi; Polyketide synthase (PKS); Stress resistance.

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References

    1. Cary JW, Harris-Coward PY, Ehrlich KC, Di Mavungu JD, Malysheva SV, De Saeger S, Dowd PF, Shantappa S, Martens SL, Calvo AM (2014) Functional characterization of a veA-dependent polyketide synthase gene in Aspergillus flavus necessary for the synthesis of asparasone, a sclerotium-specific pigment. Fungal Genet Biol : FG & B 64:25–35. https://doi.org/10.1016/j.fgb.2014.01.001 - DOI
    1. Casadevall A (2018) Melanin triggers antifungal defences. Nature 555(7696):319–320. https://doi.org/10.1038/d41586-018-02370-x - DOI - PubMed
    1. Chang PK, Cary JW, Lebar MD (2020) Biosynthesis of conidial and sclerotial pigments in Aspergillus species. Appl Microbiol Biotechnol 104(6):2277–2286. https://doi.org/10.1007/s00253-020-10347-y - DOI - PubMed
    1. Chen YX, Feng P, Shang YF, Xu YJ, Wang CS (2015) Biosynthesis of non-melanin pigment by a divergent polyketide synthase in Metarhizium robertsii. Fungal Genet Biol : FG & B 81:142–149. https://doi.org/10.1016/j.fgb.2014.10.018 - DOI
    1. Chen MB, Liu QK, Gao SS, Young AE, Jacobsen SE, Tang Y (2019) Genome mining and biosynthesis of a polyketide from a biofertilizer fungus that can facilitate reductive iron assimilation in plant. Proc N Acad Sci U S A 116(12):5499–5504. https://doi.org/10.1073/pnas.1819998116 - DOI

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