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Published Erratum
. 2024 Sep 18;14(1):21823.
doi: 10.1038/s41598-024-71815-3.

Author Correction: Melatonin-mediated mitophagy protects against early brain injury after subarachnoid hemorrhage through inhibition of NLRP3 inflammasome activation

Shenglong Cao #  1 , Sudeep Shrestha #  1 , Jianru Li  1 , Xiaobo Yu  1 , Jingyin Chen  1 , Feng Yan  1 , Guangyu Ying  1 , Chi Gu  1 , Lin Wang  1 , Gao Chen  2
Affiliations
Published Erratum

Author Correction: Melatonin-mediated mitophagy protects against early brain injury after subarachnoid hemorrhage through inhibition of NLRP3 inflammasome activation

Shenglong Cao et al. Sci Rep. .
No abstract available

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Figures

Figure 2
Figure 2
Effect of melatonin treatment on autophagy. (A) The Western blot relative band density of LC3; n = 6 per group. (B) The Western blot relative band density of Atg5; n = 6 per group. The cropped bands had been run under the same experimental conditions. (C) Representative micrographs showing double immunofluorescence labeling of Beclin-1 with NeuN (neuronal marker), GFAP (astrocyte marker) and Iba-1 (microglia marker) in the ipsilateral basal cortex at 24 h after SAH. White arrows indicated double-labeled cells. Scale bar = 50 μm. *P < 0.05 versus sham group, #P < 0.05 versus SAH + vehicle group, and &P < 0.05 versus SAH + Mel group.
Figure 3
Figure 3
Effect of melatonin treatment on mitophagy and ROS generation. (A) The Western blot relative band density of Parkin; n = 6 per group. (B) The Western blot relative band density of PINK1; n = 6 per group. The cropped bands had been run under the same experimental conditions. (C) Ultrastructural changes in mitochondria in the ipsilateral basal cortex at 24 h after SAH induction. (D) The quantification of ROS levels in the ipsilateral basal cortex at 24 h after SAH; n = 6 per group. *P < 0.05 versus sham group, #P < 0.05 versus SAH + vehicle group, and &P < 0.05 versus SAH + Mel group.
Figure 4
Figure 4
Melatonin treatment inhibits NLRP3 inflammasome activation. (A) Representative Western blot bands of NLRP3, ASC, and cleaved caspase-1. (B–D) The relative band densities of NLRP3, ASC, and cleaved caspase-1; n = 6 per group. The cropped bands had been run under the same experimental conditions. *P < 0.05 versus sham group, #P < 0.05 versus SAH + vehicle group, and &P < 0.05 versus SAH + Mel group.
Figure 5
Figure 5
Melatonin treatment inhibits microglia activation and pro-inflammatory cytokine secretion. (A) The Western blot relative band density of IL-1β; n = 6 per group. (B) The Western blot relative band density of IL-18; n = 6 per group. The cropped bands had been run under the same experimental conditions. (C) Upper: representative micrographs showing microglial activation in the lesion area at 24 h after SAH, with immunofluorescence for Iba-1. Upper scale bar = 50 μm, lower scale bar = 10 μm. Lower: quantification of Iba-1-positive cells. *P < 0.05 versus sham group, #P < 0.05 versus SAH + vehicle group, and &P < 0.05 versus SAH + Mel group.
Figure 6
Figure 6
Effect of melatonin treatment on neuronal cell death. (A) Representative micrographs showing TUNEL, PANT and Fluoro-Jade C staining in the ipsilateral basal cortex at 24 h after SAH. Scale bar = 50 μm. (B–D) The quantification of TUNEL-positive cells, PANT-positive cells, and Fluoro-Jade C-positive cells. *P < 0.05 versus sham group, #P < 0.05 versus SAH + vehicle group, and &P < 0.05 versus SAH + Mel group.
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