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. 2024 Sep 17;44(10):63.
doi: 10.1007/s11032-024-01498-1. eCollection 2024 Oct.

Identification and mapping of late blight resistance QTLs in the wild tomato accession PI 224710 (Solanum pimpinellifolium)

Sihui Gao  1   2 , Majid R Foolad  1
Affiliations

Identification and mapping of late blight resistance QTLs in the wild tomato accession PI 224710 (Solanum pimpinellifolium)

Sihui Gao et al. Mol Breed. .

Abstract

Late blight (LB), caused by oomycete Phytophthora infestans, is one of the most destructive diseases of the cultivated tomato, Solanum lycopersicum. Since new and aggressive clonal lineages of P. infestans, many of which overcoming formerly effective fungicides or host resistance genes, have continued to emerge, it is crucial to identify, characterize, and utilize new sources of host resistance in tomato breeding. A recent screening of tomato germplasm identified Solanum pimpinellifolium accession PI 224710 with very strong resistance to several current P. infestans clonal lineages. The present study aimed to identify and characterize QTLs associated with LB resistance in PI 224710. Disease screening of a large F2 population (n = 1721), derived from a cross between PI 224710 and LB-susceptible tomato breeding line Fla. 8059, followed by F3 progeny testing, resulted in the identification of 43 highly-resistant and 27 highly-susceptible F2 individuals. A selective genotyping approach, using 469 non-identical SNP markers, resulted in the construction of a genetic linkage map and identification of three LB-resistance QTLs on chromosomes 6, 9 and 10 of PI 224710. A comparison of the QTLs genomic locations with the tomato physical map resulted in the identification of several candidate genes, which might be underpinning the LB-resistance QTLs in PI 224710. The identified markers associated with the LB-resistance QTLs can be utilized in breeding programs to transfer resistance from PI 224710 into tomato breeding lines and hybrid cultivars via marker-assisted breeding; they also can be used to develop near-isogenic lines for fine mapping of the QTLs.

Supplementary information: The online version contains supplementary material available at 10.1007/s11032-024-01498-1.

Keywords: Disease Resistance; Phytophthora infestans; QTL Mapping; SNP markers; Selective Genotyping; Solanum lycopersicum; Trait-based Analysis; Vertical Resistance.

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Conflict of interest statement

Competing interestThe authors declare no competing interest.

Figures

Fig. 1
Fig. 1
Frequency distribution of the percentage disease severity (%DS) in the original F2 population (n = 1721 individuals), derived from a cross between LB-susceptible breeding line Fla. 8059 and LB-resistant Solanum pimpinellfolium accession PI 224710
Fig. 2
Fig. 2
A genetic linkage map of tomato developed based on 469 SNP markers and 70 selected F2 individuals (43 LB-resistant and 27 LB-susceptible) derived from a cross between breeding line Fla. 8059 and Solanum pimpinellifolium accession PI 224710. The genetic map was constructed using JoinMap 4.1 and visually represented by MapChart 2.3
Fig. 3
Fig. 3
Late blight resistance QTLs detected in Solanum pimpinellifolium accession PI 224710. X-axis represents LOD score generated by CIM likelihood analysis, and Y-axis represents SNP markers on the genetic map. This graph is generated by QGene 4.4.0
See this image and copyright information in PMC

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