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. 2024 Nov;598(21):2670-2682.
doi: 10.1002/1873-3468.15020. Epub 2024 Sep 19.

The splicing factor hnRNPL demonstrates conserved myocardial regulation across species and is altered in heart failure

Isabelle Draper  1 , Wanting Huang  1 , Suchita Pande  1 , Aaron Zou  1   2 , Timothy D Calamaras  1 , Richard H Choe  1 , Ana Correia-Branco  2 , Ariel L Mei  1 , Howard H Chen  1 , Hannah R Littel  3 , Mekala Gunasekaran  3 , Natalya M Wells  3 , Christine C Bruels  3 , Audrey L Daugherty  3 , Matthew J Wolf  4 , Peter B Kang  3 , Vicky K Yang  5 , Donna K Slonim  6 , Mary C Wallingford  2 , Robert M Blanton  1
Affiliations

The splicing factor hnRNPL demonstrates conserved myocardial regulation across species and is altered in heart failure

Isabelle Draper et al. FEBS Lett. 2024 Nov.

Abstract

Heart failure (HF) is highly prevalent. Mechanisms underlying HF remain incompletely understood. Splicing factors (SF), which control pre-mRNA alternative splicing, regulate cardiac structure and function. This study investigated regulation of the splicing factor heterogeneous nuclear ribonucleoprotein-L (hnRNPL) in the failing heart. hnRNPL protein increased in left ventricular tissue from mice with transaortic constriction-induced HF and from HF patients. In left ventricular tissue, hnRNPL was detected predominantly in nuclei. Knockdown of the hnRNPL homolog Smooth in Drosophila induced cardiomyopathy. Computational analysis of predicted mouse and human hnRNPL binding sites suggested hnRNPL-mediated alternative splicing of tropomyosin, which was confirmed in C2C12 myoblasts. These findings identify hnRNPL as a sensor of cardiac dysfunction and suggest that disturbances of hnRNPL affect alternative splicing in HF.

Keywords: cardiomyopathy; heart failure; hnRNPL; mRNA splicing; splicing factors.

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Conflict of interest statement

CONFLICTS OF INTEREST & ETHICS STATEMENT

The authors declare no conflicts of interest.

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