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. 2024 Oct;17(10):e011846.
doi: 10.1161/CIRCHEARTFAILURE.124.011846. Epub 2024 Sep 20.

Infarct Size Reduction With Cyclosporine A in Circulatory Death Rat Hearts: Reducing Effective Ischemia Time With Therapy During Reperfusion

Zachary Kiernan  1 , Gina Labate  1 , Qun Chen  2 , Edward J Lesnefsky  3   4 , Mohammed Quader  1   4
Affiliations

Infarct Size Reduction With Cyclosporine A in Circulatory Death Rat Hearts: Reducing Effective Ischemia Time With Therapy During Reperfusion

Zachary Kiernan et al. Circ Heart Fail. 2024 Oct.
No abstract available

Keywords: calcium; cyclosporine; mitochondrial permeability transition pore; reperfusion injury; warm ischemia.

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Conflict of interest statement

None.

Figures

Figure.
Figure.
Infarct Size Reduction Following Cyclosporine A Treatment in DCD Rat Hearts with Prolonged Warm Ischemia Time. Cyclosporine A (CyA) significantly reduced average infarct size in hearts after 25 minutes (n=10) (14.6±1.4% vs. 25.4±3.3%, p = 0.0148), 30 minutes (n=10) (26.6±2.2% vs. 33.7±1.6%, p = 0.0383), and 35 minutes (n=10) (26.6±1.6 vs. 39.0±1.6%, p = 0.0076) of ischemia when compared to untreated hearts of identical warm ischemia times (WIT) (n=8–10 per group) (Panel A). To correlate the reduced infarct sizes measured in CyA-treated hearts with a new “perceived” ischemia time, a reference plot was generated by measuring infarct size in untreated hearts at varying ischemia times ranging from 0 minutes to 35 minutes, at 5 minute intervals (Panel B; black circles) (n=5–10 per group). Using linear regression, a trendline was then generated (y = 0.7836x + 9.860, where ‘y’ represents infarct size and ‘x’ represents ischemia time). The average infarct sizes measured from CyA-treated hearts after 25, 30 and 35 minutes of ischemia were then substituted for ‘y’, solving for ‘x’, or the new “perceived” ischemia time. The new “perceived” ischemia times for CyA-treated hearts following 25, 30 and 35 minutes of induced ischemia were 6, 21, and 21 minutes, respectively (Panel B; colored shapes). For specific comparisons between CyA-treated and untreated hearts, an unpaired T-test was employed, and data are presented as mean ± SEM, * = P <0.05.
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References

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