Glomerular immune deposits in kidneys from patients with no clinical or light microscopic evidence of glomerulonephritis. Assessment of the influence of autolysis on identification of immunoglobulins and complement

Abstract

Using a direct fluorescent staining technique, immunofluorescent microscopy (IFM) demonstrated glomerular deposits of IgG and IgM and/or fractions of complement in kidney tissue from 24% of 33 patients examined post mortem and in 39% of kidney biopsies obtained from 23 patients on lithium treatment. All the patients investigated had a normal blood pressure. There was no evidence of glomerulonephritis (GN) neither clinically, at light microscopy, nor on laboratory investigation. These "spontaneously" deposited immunoglobulins and complement fractions in glomeruli will obviously by demonstrated in kidney biopsies from patients with GN, even though they bear no relation to the disease. This will therefore preclude an immunopathological classification which relates to histological and clinical findings. A control study of the IFM findings in glomeruli on 13 surgically removed kidneys showed optimal identification and no further glomerular deposition of immunoglobulins during the 72 hours following nephrectomy, at temperatures below 10 degrees C. Clq and C3 were less stable and were only demonstrated with certainty up to 24 hours after nephrectomy.