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. 2024 Sep 23;24(1):1175.
doi: 10.1186/s12885-024-12896-1.

Expression profiling of circulating lncRNA GIAT4RA, lncRNA AATBC, lncRNA Sirt1-AS, and SMARCB1 in lung cancer patients

Neveen A Hussein  1 , Samia A Ebied  2 , Abdel Aziz M Belal  3 , Mohamad A Ahmad  4 , El Sayed A Weheida  2
Affiliations

Expression profiling of circulating lncRNA GIAT4RA, lncRNA AATBC, lncRNA Sirt1-AS, and SMARCB1 in lung cancer patients

Neveen A Hussein et al. BMC Cancer. .

Abstract

Long noncoding RNAs (lncRNAs) are crucial regulators of biological processes such as transcription interference and activation, chromatin remodeling, and mRNA translation. Uncontrolled gene expression could result from various epigenetic modifiers, like lncRNAs. So, this study aimed to evaluate the expression profiles of lncRNA GIAT4RA, lncRNA AATBC, lncRNA Sirt1-AS, and SMARCB1 in lung cancer. The current study included lung cancer patients (n = 50), patients with chronic inflammatory diseases (n = 30), and healthy volunteers (n = 20). The expression of blood genes and the concentration of serum neuron-specific enolase were determined by real-time PCR and electrochemiluminescence immunoassay, respectively. The receiver operating characteristic and Kaplan-Meier analyses assess the sensitivity of genes as diagnostic and prognostic biomarkers, respectively. LncRNA GIAT4RA and lncRNA AATBC were upregulated, while lncRNA Sirt1-AS was significantly downregulated in all patients compared to the control group. SMARCB1 expression was significantly downregulated in chronic inflammatory patients, while in those with lung cancer, it showed an insignificant difference. The expression of lncRNA GIAT4RA and lncRNA AATBC was significantly related to the stage of lung cancer. The survival analyses showed that lower lncRNA Sirt1-AS was linked to lung cancer patients' poorer disease-free survival and overall survival. Differences in lncRNA GIAT4RA, lncRNA AATBC, and lncRNA Sirt1-AS expression were detected in all patients. The consequent abnormal expression of lncRNAs could be crucial in lung cancer development. LncRNA GIAT4RA, lncRNA AATBC, and lncRNA Sirt1-AS may be utilized as promising diagnostic biomarkers. LncRNA AATBC, lncRNA Sirt1-AS, and SMARCB1 may be valuable prognostic biomarkers for lung cancer.

Keywords: Lung cancer; SMARCB1; lncRNA AATBC; lncRNA GIAT4RA; lncRNA Sirt1-AS.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
LncRNA GIAT4RA, lncRNA AATBC, lncRNA Sirt1-AS, SMARCB1 expression, and NSE concentration in control and patients. * P < 0.05
Fig. 2
Fig. 2
LncRNA GIAT4RA, lncRNA AATBC, lncRNA Sirt1-AS, and SMARCB1 correlations in lung cancer patients
Fig. 3
Fig. 3
LncRNA GIAT4RA, lncRNA AATBC, lncRNA Sirt1-AS, and SMARCB1 relations with smoking and gender of chronic inflammatory patients
Fig. 4
Fig. 4
LncRNA GIAT4RA, lncRNA AATBC, lncRNA Sirt1-AS, and SMARCB1 relations with characteristics of lung cancer patients
Fig. 5
Fig. 5
ROC curves for lncRNA GIAT4RA, lncRNA AATBC, lncRNA Sirt1-AS, SMARCB1 and NSE protein in (A) lung cancer, and (B) chronic inflammatory patients
Fig. 6
Fig. 6
Kaplan-meier survival curves for disease free survival of lncRNA GIAT4RA, lncRNA AATBC, lncRNA Sirt1-AS, SMARCB1 expression, and NSE in lung cancer patients
Fig. 7
Fig. 7
Kaplan-meier survival curves for overall survival of lncRNA GIAT4RA, lncRNA AATBC, lncRNA Sirt1-AS, SMARCB1 expression, and NSE in lung cancer patients
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