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. 2024 Nov:91:105898.
doi: 10.1016/j.msard.2024.105898. Epub 2024 Sep 17.

Profiling of B cells and their subsets by whole blood gene expression analysis versus flow cytometry in multiple sclerosis

Sahla El Mahdaoui  1 , Marina Rode von Essen  2 , Marie Mathilde Hansen  2 , Jeppe Romme Christensen  2 , Finn Sellebjerg  3 , Helle Bach Søndergaard  2
Affiliations
Free article

Profiling of B cells and their subsets by whole blood gene expression analysis versus flow cytometry in multiple sclerosis

Sahla El Mahdaoui et al. Mult Scler Relat Disord. 2024 Nov.
Free article

Abstract

We investigated if differentially expressed mRNA targets could be used as surrogate markers for circulating B cells and subsets. In paired blood samples from patients with untreated, anti-CD20-treated, fingolimod-treated, and natalizumab-treated multiple sclerosis, whole blood expression of CD19 correlated with B cell counts determined by flow cytometry, ROR1 with transitional B cells, TCL1A and ZNF727 with naïve B cells, NEXMIF with memory B cells and BCMA with plasmablasts. CD19 expression distinguished patients with B cell repletion and may be used as an alternative to flow cytometry, but NEXMIF was unsuitable for memory B cell monitoring in rituximab-treated patients.

Keywords: Anti-CD20; Cell sorting; Extended interval dosing; Infusion; Microarray.

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Conflict of interest statement

Declaration of competing interest SEM has received speaker honoraria and non-financial support for conference participation from Merck. MRvE has received speaker honoraria from Merck. MH has received non-financial support for conference participation from Merck and Sanofi. JRC has received speaker honoraria from Biogen. FS has served on scientific advisory boards for, served as consultant for, received support for congress participation or received speaker honoraria from Alexion, Biogen, Bristol Myers Squibb, Lundbeck, Merck, Novartis, Roche and Sanofi Genzyme. His laboratory has received research support from Biogen, Merck, Novartis, Roche and Sanofi Genzyme. HBS reports no conflicts of interest.

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