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. 2024 Sep 11:15:1439522.
doi: 10.3389/fmicb.2024.1439522. eCollection 2024.

Antimicrobial resistance and genomic analysis of Vibrio parahaemolyticus isolates from foodborne outbreaks, Huzhou, China, 2019-2023

Wei Yan #  1 , Lei Ji #  1 , Fenfen Dong  1 , Liping Chen  1 , Rui Yuan  1 , Peng Zhang  1
Affiliations

Antimicrobial resistance and genomic analysis of Vibrio parahaemolyticus isolates from foodborne outbreaks, Huzhou, China, 2019-2023

Wei Yan et al. Front Microbiol. .

Abstract

Objective: The purpose of this study was to investigate the epidemiological and genomic characteristics of Vibrio parahaemolyticus (V. parahaemolyticus) isolates from outbreaks in Huzhou, China.

Methods: This study aims to analyze the epidemiological data on V. parahaemolyticus outbreaks reported in Huzhou from 2019 to 2023. A total of 70 V. parahaemolyticus outbreak isolates were collected. The antibiotic resistance, serotypes, molecular typing, and genomic characteristics of these isolates were analyzed.

Results: Most outbreaks of V. parahaemolyticus infection occurred in the summer, and the majority of outbreaks occurred in restaurants and rural banquets. High resistance rates were observed for ampicillin (AMP, 24.29%), followed by tetracycline (TET, 15.71%) and trimethoprim-sulfamethoxazole (SXT, 15.71%). The newly emerged serotype O10:K4 became dominant from 2021 to 2023, with most isolates belonging to ST3. The resistance gene blaCARB was frequently detected among these isolates. The pulsed-field gel electrophoresis (PFGE) and whole-genome single-nucleotide polymorphisms (wgSNPs) effectively differentiated the nine outbreaks.

Conclusion: The newly emerged serotype O10:K4 became dominant from 2021 to 2023, with most isolates being ST3. PFGE and WGS technologies provided reliable methods for typing and identifying V. parahaemolyticus for outbreaks.

Keywords: PFGE; Vibrio parahaemolyticus; WGS; foodborne disease; outbreak.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1
PFGE pattern of 70 Vibrio parahaemolyticus isolates from outbreaks.
Figure 2
Figure 2
Annotation heatmap of virulence factors among 70 V. parahaemolyticus isolates from outbreaks.
Figure 3
Figure 3
Phylogenetic analysis of all 70 V. parahaemolyticus isolates from outbreaks, 2019–2023.
Figure 4
Figure 4
Phylogenetic analysis of 54 V. parahaemolyticus isolates from clade I.
See this image and copyright information in PMC

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