Genetic Analysis of Vitamin C Content in Rapeseed Seedlings by the Major Gene Plus Polygene Mixed Effect Model

Abstract

Rapeseed (Brassica napus L.) seedlings are rich in vitamin C (Vc), which is beneficial for humans. Understanding the genetic variance in Vc content has practical significance for the breeding of "oil-vegetable dual-purpose" rapeseed. In this study, the joint segregation analysis of a mixed genetic model of the major gene plus polygene was conducted on the Vc content in rapeseed seedlings. Six generations, including two parents, P₁ (high Vc content) and P₂ (low Vc content), F₁, and the populations of F₂, BC₁P1, and BC₁P₂ from two crosses were investigated. Genetic analysis revealed that the genetic model MX2-A-AD was the most fitting genetic model, which indicates that Vc content is controlled by two additive major genes plus additive and dominance polygenes. In addition, the whole heritability in F₂ and BC₁P1 was higher than that in BC₁P2. The largest coefficient of variation for Vc content appeared in the F₂ generation. Therefore, for Vc content, the method of single cross recross or single backcross are suggested to transfer major genes, and the selection in F₂ would be more efficient than that in other generations. Our findings provide a theoretical basis for the quantitative trait locus (QTL) mapping and breeding of Vc content in rapeseed seedlings.

Keywords: inheritance; major gene; polygene; rapeseed seedlings; vitamin C.

Figure 1

Comparison of Vc content among the parents (P₁ and P₂) and F₁ generation of crosses A and B. Different letters above the bars indicate significant differences (p < 0.05) as determined by a one-way ANOVA test. FW, fresh weight.

Figure 2

Frequency distributions of style Vc content in four generations of two crosses. (a) Cross A (8S079 × 8S084); (b) cross B (8S243 × 8S007). FW, fresh weight.