EDC-3 and EDC-4 regulate embryonic mRNA clearance and biomolecular condensate specialization

Abstract

Animal development is dictated by the selective and timely decay of mRNAs in developmental transitions, but the impact of mRNA decapping scaffold proteins in development is unclear. This study unveils the roles and interactions of the DCAP-2 decapping scaffolds EDC-3 and EDC-4 in the embryonic development of C. elegans. EDC-3 facilitates the timely removal of specific embryonic mRNAs, including cgh-1, car-1, and ifet-1 by reducing their expression and preventing excessive accumulation of DCAP-2 condensates in somatic cells. We further uncover a role for EDC-3 in defining the boundaries between P bodies, germ granules, and stress granules. Finally, we show that EDC-4 counteracts EDC-3 and engenders the assembly of DCAP-2 with the GID (CTLH) complex, a ubiquitin ligase involved in maternal-to-zygotic transition (MZT). Our findings support a model where multiple RNA decay mechanisms temporally clear maternal and zygotic mRNAs throughout embryonic development.

Keywords: C. elegans;; CP: Developmental biology; CP: Molecular biology; DCAP-2/Dcp2; EDC-3; EDC-4; GID/CTLH complex; IFET-1/CAR-1/CGH-1 complex; P bodies; decapping; germ granules; mRNA decay; microRNA.