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. 2024 Sep 20;13(9):1135.
doi: 10.3390/antiox13091135.

Evaluation of the Protective Role of Vitamin E against ROS-Driven Lipid Oxidation in Model Cell Membranes

Dilara Kilicarslan You  1 , Ahmed Fuwad  2   3 , Ki Hyok Lee  4 , Hyung Kyo Kim  4 , Lifeng Kang  5 , Sun Min Kim  1   2   6 , Tae-Joon Jeon  1   5   6   7
Affiliations

Evaluation of the Protective Role of Vitamin E against ROS-Driven Lipid Oxidation in Model Cell Membranes

Dilara Kilicarslan You et al. Antioxidants (Basel). .

Abstract

Reactive oxygen species (ROS) are chemically reactive oxygen-containing compounds generated by various factors in the body. Antioxidants mitigate the damaging effects of ROS by playing a critical role in regulating redox balance and signaling. In this study, the interplay between reactive oxygen species (ROS) and antioxidants in the context of lipid dynamics were investigated. The interaction between hydrogen peroxide (H2O2) as an ROS and vitamin E (α-tocopherol) as an antioxidant was examined. Model membranes containing both saturated and unsaturated lipids served as experimental platforms to investigate the influence of H2O2 on phospholipid unsaturation and the role of antioxidants in this process. The results demonstrated that H2O2 has a negative effect on membrane stability and disrupts the lipid membrane structure, whereas the presence of antioxidants protects the lipid membrane from the detrimental effects of ROS. The model membranes used here are a useful tool for understanding ROS-antioxidant interactions at the molecular level in vitro.

Keywords: antioxidants; hydrogen peroxide; lipid membranes; lipid oxidation; reactive oxygen species; vitamin E.

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Conflict of interest statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Figure 1
Figure 1
(A) Size distribution of DOPC, POPC, and DMPC liposomes with and without vitamin E and H2O2. (B) Zeta potential analysis of the DOPC, POPC, and DMPC liposomes interaction with and without vitamin E and H2O2. DOPC: 1,2-dioleoyl-sn-glycero-3-phosphocholine; POPC: 1-pal-mitoyl-2-oleoyl-glycero-3-phosphocholine; DMPC: 1,2-dimyristoyl-sn-glycero-3-phosphocholine. Error bars represent the means ± SEM of n = 4. Statistical significance was determined using a two-tailed t-test, * p < 0.05, compared with the control group.
Figure 2
Figure 2
Structural characterization of the lipid membrane and vitamin E-H2O2 combination with lipid mixtures using (A) DOPC, (B) POPC, and (C) DMPC. FTIR: Fourier transform infrared spectroscopy, DOPC: 1,2-dioleoyl-sn-glycero-3-phosphocholine; POPC: 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine; DMPC: 1,2-dimyristoyl-sn-glycero-3-phosphocholine.
Figure 3
Figure 3
The results of the spectral subtraction analysis of the methyl and methylene bands of the lipid membrane and vitamin E-H2O2 combination with lipid mixtures using (A) DOPC, (B) POPC, and (C) DMPC. FTIR: Fourier transform infrared spectroscopy, DOPC: 1,2-dioleoyl-sn-glycero-3-phosphocholine; POPC: 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine; DMPC: 1,2-dimyristoyl-sn-glycero-3-phosphocholine.
Figure 4
Figure 4
(A) Schematic representation of fluorescence intensity analysis of liposomes containing NBD-PE. Liposome fluorescence spectrum of (B) DOPC, (C) POPC, and (D) DMPC in different conditions. DOPC: 1,2-dioleoyl-sn-glycero-3-phosphocholine; POPC: 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine; DMPC: 1,2-dimyristoyl-sn-glycero-3-phosphocholine; NBD-PE: 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl).
Figure 5
Figure 5
Fluorescence images of GUVs labeled with NBD (green). (a) DOPC-NBD, (b) POPC-NBD-PE, and (c) DMPC-NBD-PE GUVs and fluorescence intensity measurements. Fluorescence intensity was measured using ImageJ software along the colored dashed lines (a–c) for the three lipid groups. GUVs: giant unilamellar vesicles; DOPC: 1,2-dioleoyl-sn-glycero-3-phosphocholine; POPC: 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine; DMPC: 1,2-dimyristoyl-sn-glycero-3-phosphocholine; NBD-PE: 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl). Scale bars = 20 µm.
Figure 6
Figure 6
Electrical measurements of vitamin E and H2O2 flux on (A) DOPC, (B) POPC, and (C) DMPC BLMs. Lipid bilayer thickness was measured in the presence and absence of H2O2 and vitamin E with DOPC, POPC, and DMPC. DOPC: 1,2-dioleoyl-sn-glycero-3-phosphocholine; POPC: 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine; DMPC: 1,2-dimyristoyl-sn-glycero-3-phosphocholine. Error bars represent the means ± SEM of n = 6. Statistical significance was determined using a two-tailed t-test, * p < 0.05, compared with the control samples.
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References

    1. Moloney J.N., Cotter T.G. ROS Signalling in the Biology of Cancer. Semin. Cell Dev. Biol. 2018;80:50–64. doi: 10.1016/j.semcdb.2017.05.023. - DOI - PubMed
    1. Andrés C.M.C., Pérez de la Lastra J.M., Juan C.A., Plou F.J., Pérez-Lebeña E. Chemistry of Hydrogen Peroxide Formation and Elimination in Mammalian Cells, and Its Role in Various Pathologies. Stresses. 2022;2:256–274. doi: 10.3390/stresses2030019. - DOI
    1. Park C., Kim D.H., Kim T.H., Jeong S.U., Yoon J.H., Moon S.K., Kwon C.Y., Park S.H., Hong S.H., Shim J.H., et al. Improvement of Oxidative Stress-Induced Cytotoxicity of Angelica Keiskei (Miq.) Koidz. Leaves Extract through Activation of Heme Oxygenase-1 in C2C12 Murine Myoblasts. Biotechnol. Bioprocess Eng. 2023;28:51–62. doi: 10.1007/s12257-022-0310-7. - DOI
    1. Zhu Y., Cheng Y., Ma L. Circ_0005280 Protects Human Lens Epithelial Cells against H2O2-Induced Apoptosis and Oxidative Stress Though MiR-326/PLCD3 Axis. Biotechnol. Bioprocess Eng. 2023;28:101–111. doi: 10.1007/s12257-021-0197-8. - DOI
    1. Mohd Mutalip S.S., Ab-Rahim S., Rajikin M.H. Vitamin E as an Antioxidant in Female Reproductive Health. Antioxidants. 2018;7:22. doi: 10.3390/antiox7020022. - DOI - PMC - PubMed

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