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. 2024 Sep 14;13(9):885.
doi: 10.3390/antibiotics13090885.

Evaluation of T2 Magnetic Resonance (T2MR®) Technology for the Early Detection of ESKAPEc Pathogens in Septic Patients

Celestino Bonura  1 , Domenico Graceffa  2 , Salvatore Distefano  2 , Simona De Grazia  1 , Oscar Guzman  3 , Brian Bohn  3 , Mariachiara Ippolito  4   5 , Salvatore Campanella  4 , Angelica Ancona  4 , Marta Caputo  4 , Pietro Mirasola  4 , Cesira Palmeri  4   5 , Santi Maurizio Raineri  4   5 , Antonino Giarratano  4   5 , Giovanni Maurizio Giammanco  1 , Andrea Cortegiani  4   5
Affiliations

Evaluation of T2 Magnetic Resonance (T2MR®) Technology for the Early Detection of ESKAPEc Pathogens in Septic Patients

Celestino Bonura et al. Antibiotics (Basel). .

Abstract

Bloodstream infections (BSIs) and sepsis are a major cause of morbidity and mortality. Appropriate early antibiotic therapy is crucial for improving the survival of patients with sepsis and septic shock. T2 magnetic resonance (T2MR®) technology may enable fast and sensitive detection of ESKAPEc pathogens directly from whole-blood samples. We aimed to evaluate concordance between the T2Bacteria® Panel and standard blood culture and its impact on antibiotic therapy decisions. We conducted a single-centre retrospective study on patients with sepsis-induced hypotension or septic shock admitted to general, post-operative/neurosurgical, and cardiothoracic Intensive Care Units who were tested with the T2Bacteria® Panel from January 2021 to December 2022. Eighty-five consecutively admitted patients were included, for a total of 85 paired tests. A total of 48 ESKAPEc pathogens were identified by the T2Bacteria® Panel. The concordance rate between the T2Bacteria® Panel and blood cultures was 81% (69/85), with 20 concordant-positive and 49 concordant-negative cases. For the 25 microorganisms grown from accompanying blood cultures, blood pathogen coverage by the T2Bacteria® Panel was 88%. In this cohort of severely ill septic patients, the T2Bacteria® Panel was highly concordant and was able to detect more ESKAPEc pathogens, with a significantly shorter turn-around time compared to conventional blood cultures. The T2Bacteria® Panel also significantly impacted decisions on antibiotic therapy.

Keywords: antimicrobial stewardship; bacteraemia; sepsis.

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Conflict of interest statement

B.C.B. and O.E.G. are employees and shareholders of T2 Biosystems Inc. The other authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
T2Bacteria® Panel concordance with blood culture results (excluding off-panel organisms).
Figure 2
Figure 2
Blood pathogen coverage by T2Bacteria® Panel (excluding contaminants).
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