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. 2024 Sep 19;12(9):2125.
doi: 10.3390/biomedicines12092125.

Characteristics of Mucosa-Associated Microbiota in Ulcerative Colitis Patients with 5-Aminosalicylic Acid Intolerance

Hiroshi Matsumoto  1 , Momoyo Sasahira  1 , Tei Tei Go  1 , Shogen Yo  1 , Takehiro Ninomiya  1 , Motoyasu Osawa  1 , Osamu Handa  1 , Eiji Umegami  1 , Ryo Inoue  2 , Akiko Shiotani  1
Affiliations

Characteristics of Mucosa-Associated Microbiota in Ulcerative Colitis Patients with 5-Aminosalicylic Acid Intolerance

Hiroshi Matsumoto et al. Biomedicines. .

Abstract

Background/objectives: 5-Aminosalicylic acid (5-ASA) is a first-line therapy for ulcerative colitis (UC). This study examined the mucosa-associated microbiota (MAM) in UC patients, distinguishing between those who were 5-ASA tolerant and intolerant.

Methods: Brushing samples were collected from the sigmoid and ileal end of patients with UC during endoscopic procedures. The samples were profiled by using the Illumina MiSeq platform. The V3-V4 regions of the 16S rRNA gene (460 bp) were amplified by using tailed PCR.

Results: A total of 15 patients with 5-ASA intolerance, 38 patients with 5-ASA tolerance, and 19 healthy controls were recruited in this study. The α-diversity indices were remarkably different among the three groups in the ileum mucosa but not in the sigmoid colon. In the ileum mucosa, Alistipes, Ruminococcaceae, and Odoribacter were less abundant in the 5-ASA-intolerant group than in the control and 5-ASA-tolerant groups. On the contrary, Merdibacter, Brevundimonas, and Porphyromonas were more abundant in the 5-ASA-intolerant group than in other groups.

Conclusions: The present study showed that the changes in MAM were characterized by a decrease in mucoprotective bacteria rather than an increase in harmful bacteria.

Keywords: 5-ASA intolerance; dysbiosis; gut microbiota; mucosa-associated microbiota; ulcerative colitis.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 5
Figure 5
Comparison between two groups of ileal MAM. Bacteria species that showed significant differences between the two groups of (A) control (cyan bar) and 5-ASA tolerance (yellow green bar), (B) control and 5-ASA intolerance (blue bar), (C) 5-ASA intolerance and tolerance.
Figure 5
Figure 5
Comparison between two groups of ileal MAM. Bacteria species that showed significant differences between the two groups of (A) control (cyan bar) and 5-ASA tolerance (yellow green bar), (B) control and 5-ASA intolerance (blue bar), (C) 5-ASA intolerance and tolerance.
Figure 1
Figure 1
α-Diversity of ileal mucosa-associated microbiota (MAM); (A) observed features, (B) Shannon entropy, (C) Chao1.
Figure 2
Figure 2
Taxonomic analysis of mucosa-associated microbiota (MAM) analysis using ileum mucus.
Figure 3
Figure 3
Comparison of three groups of ileal MAM: list of bacterial species that showed significant differences among three groups. The abundance of five intolerance groups was lower than the other two groups. (A) Merdibacter, (B) Brevundimonas, and (C) Porphymonas.
Figure 4
Figure 4
Comparison of three groups of ileal MAM: list of bacterial species that showed significant differences among three groups. The abundance of five intolerance groups was higher than the other two groups. (A) Alistipes, (B) Lachnospiraceae, (C) Monogiobus, (D) Ruminococcaceae, and (E) Odribacter.
Figure 6
Figure 6
α-Diversity of colonic mucosa-associated microbiota (MAM): (A) observed features, (B) Shannon entropy, and (C) Chao 1.
Figure 7
Figure 7
Taxonomic analysis of mucosa-associated microbiota (MAM) analysis using colonic mucus.
Figure 8
Figure 8
Comparison of three groups of colonic MAM: list of bacterial species that showed significant differences among three groups. The abundance of five intolerance groups was lower than the other two groups. (A) Oribacterium, (B) Laptotrichia, and (C) Sutterella.
Figure 9
Figure 9
Comparison of three groups of colonic MAM: list of bacterial species that showed significant differences among three groups. The abundance of five intolerance groups was higher than the other two groups. (A) Bifidobacterium and (B) Eggerthella.
Figure 10
Figure 10
Comparison between two groups of sigmoid colonic MAM. Bacteria species that showed significant differences between the two groups of (A) control (red bar) and 5-ASA tolerance (purple bar), (B) control and 5-ASA intolerance (orange bar), (C) 5-ASA intolerance and tolerance.
Figure 10
Figure 10
Comparison between two groups of sigmoid colonic MAM. Bacteria species that showed significant differences between the two groups of (A) control (red bar) and 5-ASA tolerance (purple bar), (B) control and 5-ASA intolerance (orange bar), (C) 5-ASA intolerance and tolerance.
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