Arntl-induced upregulation of DUSP1 inhibits tumor progression in esophageal squamous cell carcinoma by inactivating ERK signaling

Abstract

Background: Esophageal squamous cell carcinoma (ESCC) is a primary histological type of esophageal carcinoma with high morbidity. Aryl hydrocarbon receptor nuclear translocator-like (ARNTL) is a circadian clock gene associated with the progression of multiple tumors. However, its roles and mechanisms in ESCC remain unknown.

Methods: ARNTL expression was analyzed using TCGA database and detected using qRT-PCR, and ARNTL-related pathways were analyzed through GSEA. Cell functional behaviors were assessed in vitro by measuring cell viability, proliferation, and apoptosis. Cell growth in the murine model was investigated through xenograft model and immunofluorescence assays of PCNA and Ki67. The downstream targets of ARNTL were analyzed through sequencing and identified via luciferase report, ChIP, and RNA pull-down analyses. Dual-specificity protein phosphatase-1 (DUSP1) expression was analyzed using GEO datasets and measured using qRT-PCR and western blotting. Protein expression was examined via western blotting.

Results: ARNTL expression was decreased in esophageal carcinoma and associated with histological types, and elevated expression of ARNTL repressed ESCC cell viability and proliferation and facilitated cell apoptosis. ARNTL upregulation reduced tumor cell growth in murine models and decreased PCNA and Ki67 levels. Furthermore, DUSP1 was downregulated upon ARNTL silencing in ESCC. ARNTL could bind and positively regulate DUSP1 transcription. Additionally, DUSP1 silencing reversed the influences of ARNTL upregulation on cell viability, proliferation, and apoptosis in ESCC cells. ARNTL attenuated the activation of the ERK signaling by decreasing ERK phosphorylation through upregulation of DUSP1.

Conclusion: ARNTL hinders cell growth and contributes to cell apoptosis by inactivating ERK signaling through transcriptional upregulation of DUSP1 in ESCC.

Keywords: ARNTL; DUSP1; ERK; Esophageal squamous cell carcinoma; apoptosis.

Figure 1.

ARNTL is differentially expressed in ESCC.

Figure 2.

ARNTL upregulation hinders cell growth and promotes cell apoptosis in ESCC.

Figure 3.

ARNTL upregulation reduces ESCC cell growth in murine model.

Figure 4.

The potential targets of ARNTL.

Figure 5.

ARNTL regulates DUSP1 transcription.

Figure 6.

DUSP1 knockdown reverses the effect of ARNTL upregulation on cell growth in ESCC cells.

Figure 7.

DUSP1 knockdown reverses the effect of ARNTL upregulation on cell apoptosis in ESCC cells.

Figure 8.

ARNTL upregulation blocks activation of the ERK signaling by upregulating DUSP1.