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. 2024 Nov 12;13(11):e0092524.
doi: 10.1128/mra.00925-24. Epub 2024 Sep 30.

Comparative genome analysis of cluster EF bacteriophages Ajin and OverHedge isolated from soil in Tennessee

Sergei A Markov  1 , Paul Y Atuahene  1 , Clayton W Barnes  1 , Taquerra Butler  1 , Stephan D Cooper  1 , Emma M Covel  1 , Kayla R Diaz  1 , Dalon Gadson  1 , Anna C Holt  1 , Maegan A Litchfield  1 , Alexis J Nefe  1 , Comfort E Ogbu  1 , Kiara M Rupp  1 , Falisaty Simpson  1 , Elyse Wood  1
Affiliations

Comparative genome analysis of cluster EF bacteriophages Ajin and OverHedge isolated from soil in Tennessee

Sergei A Markov et al. Microbiol Resour Announc. .

Abstract

Bacteriophages Ajin and OverHedge were isolated from soil in Tennessee using the bacterium Microbacterium foliorum. Ajin and OverHedge (cluster EF) have a genome of 56,993 bp and 56,559 bp, containing 86 and 81 predicted genes, respectively. The Ajin genome has unique genes, phosphatase and glycosyltransferase, compared to the OverHedge.

Keywords: bacteriophages; genome analysis.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Fig 1
Fig 1
Transmission electron microscopy photos of bacteriophages Ajin (A) and OverHedge (B) showing a siphovirus morphology with an icosahedral capsid of diameter 52–54 nm and a 132–134 nm long flexible tail (n = 7). Bacteriophage samples were stained using 1% uranyl acetate on grids attached to Pelco Tabs (Ted Peller, Inc., Redding, CA). The Hitachi H-7650 Transmission Electron Microscope (Tokyo, Japan) was used with an accelerating voltage of 100 kV.
See this image and copyright information in PMC

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