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. 2024 Oct;19(4):2367-2376.
doi: 10.1016/j.jds.2024.07.011. Epub 2024 Jul 19.

Efficacy of antimicrobial peptide P113 oral health care products on the reduction of oral bacteria number and dental plaque formation in a randomized clinical assessment

Yi-Fan Wu  1   2 , Bor-Cheng Han  3 , Wen-Yi Lin  3 , Sin-Yu Wang  1 , Thu Ya Linn  1 , Hsueh-Wen Hsu  3 , Chih-Chieh Wen  1 , Hung-Yi Liu  2 , Yi-Hua Chen  3   4   5 , Wei-Jen Chang  1   6
Affiliations

Efficacy of antimicrobial peptide P113 oral health care products on the reduction of oral bacteria number and dental plaque formation in a randomized clinical assessment

Yi-Fan Wu et al. J Dent Sci. 2024 Oct.

Abstract

Background/purpose: Dental plaque is the main cause leading to the dental caries and periodontal diseases. The main purpose of this study was to test the efficacy of oral spray containing the antimicrobial peptide P-113 on the reduction of oral bacteria number and dental plaque formation in a randomized clinical assessment.

Materials and methods: This study was divided into two parts. In Part A, we investigated the user experiences with the P-113 containing oral spray. In part B, 14 subjects in the experimental group used the P-113-containing oral spray, while 14 subjects in the control group used a placebo without the P-113 in a 4-week clinical trial. Participants were asked to use the P-113-containing oral spray or placebo 3 times per day and 5 times per use. Moreover, 3 check-ups and 2 washouts were carried out to evaluate the DMFT score, dental plaque weight, dental plaque index, and gingival index.

Results: In part A, up to 91.8% of the subjects in the experimental group were satisfied with the use of the P-113-containing oral spray. In part B, based on our PacBio SMRT sequencing platform and DADA2 analysis, the numbers of Streptococcus and Porphyromonas in the experimental group were lower than those in the control group. In addition, decreased dental plaque weight, dental plaque index, and gingival index were all observed in the experimental group.

Conclusion: The P-113-containing oral spray has the potential to reduce the dental caries and periodontal disease-related bacteria and to control the dental plaque formation.

Keywords: Dental plaque; Oral microbiota; Oral spray; P-113; PacBio SMRT sequencing.

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Conflict of interest statement

The authors have declared that there are no competing interests.

Figures

Figure 1
Figure 1
The flow chart of part A for the questionnaire's investigation.
Figure 2
Figure 2
Flow chart of part B clinical trial. DMFT: sum of the number of decayed, missing, and filled teeth in the permanent teeth.
Figure 3
Figure 3
Improvement proportion of oral health problems. ∗P < 0.05.
Figure 4
Figure 4
Positive feelings (maintained or better) of the experimental group.
Figure 5
Figure 5
Positive feelings (maintained or better) based on age.
Figure 6
Figure 6
Positive feelings (maintained or better) based on oral health status.
Figure 7
Figure 7
(a) Changes of dental plaque scores in the experimental group. (b) Changes of dental plaque scores in the control group.
Figure 8
Figure 8
(a) Percentage changes of gingival index in the experimental group. (b) Percentage changes of gingival index scores in the control group. GI: Gingival index.
Figure 9
Figure 9
(a) Relative average abundance at the phlyum level from the experimental group. (b) Relative average abundance at the phylum level from the control group. ∗P < 0.05 and ∗∗P < 0.01, respectively.
Figure 10
Figure 10
(a) Relative average abundance at the genus level from the experimental group. (b) Relative average abundance at the genus level from the control group. ∗P < 0.05.
Figure 11
Figure 11
Effects of oral spray use-experience.
See this image and copyright information in PMC

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