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. 2024 Sep 25:18:4319-4326.
doi: 10.2147/DDDT.S480774. eCollection 2024.

Protective Effect of Fasudil on Testicular Ischemia-Reperfusion Injury in Rats

Cem Kaya  1 , Alparslan Kapisiz  1 , Sibel Eryilmaz  1 , Ramazan Karabulut  1 , Zafer Turkyilmaz  1 , Mehmet Arda Inan  2 , Gizem Yaz Aydin  3 , Kaan Sonmez  1
Affiliations

Protective Effect of Fasudil on Testicular Ischemia-Reperfusion Injury in Rats

Cem Kaya et al. Drug Des Devel Ther. .

Abstract

Background: Ischemia-reperfusion (I/R) injury to the testis can lead to organ damage, infertility, and subfertility. The goal of this study was to investigate the effects of fasudil on this devastating condition.

Methods: Thirty male Long-Evans rats were divided into five groups: a control group (no torsion), rats administered fasudil (30 mg/kg, no torsion), rats subject to ischemia with no treatment (I) (I/R injury), injured rats that received treatment 1 (T1) (I/R with 30 mg/kg fasudil before detorsion), and injured rats that received treatment 2 (T2) (I/R with 30 mg/kg fasudil after detorsion). Serum levels of TNF-ɑ and IL-6, along with tissue levels of glutathione (GSH), malondialdehyde (MDA), caspase-3, and Johnsen Tubular Biopsy Score (JTBS), were measured.

Results: Group I exhibited significantly higher levels of MDA and caspase-3 than all other groups except T2 (p ˂ 0.05). Although the difference was not statistically significant, Group T2 exhibited lower MDA and caspase-3 levels than Group I (p ˃ 0.05). Additionally, Group I displayed significantly higher TNF-ɑ and IL-6 levels, and lower GSH and JTBS values, than the other groups (p ˂ 0.05).

Conclusion: Our findings indicate that fasudil protects the testis from I/R injury, particularly when administered early.

Keywords: fasudil; ischemia/reperfusion; testis.

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Conflict of interest statement

The authors report no conflicts of interest in this work.

Figures

Figure 1
Figure 1
In the example of group T1; JTBS score 9 with mature sperms and spermatids.
Figure 2
Figure 2
In the example of ischemia group; JTBS score 5 with alive spermatocytes but dead spermatids.
See this image and copyright information in PMC

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