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. 2025:2857:45-59.
doi: 10.1007/978-1-0716-4128-6_5.

γδ T-Cell Immunophenotype for the Study of Human Aging

Anna Maria Corsale  1   2 , Marta Di Simone  1   2 , Serena Meraviglia  1   2 , Calogero Caruso  3
Affiliations

γδ T-Cell Immunophenotype for the Study of Human Aging

Anna Maria Corsale et al. Methods Mol Biol. 2025.

Abstract

Flow cytometry serves as a crucial tool in immunology, allowing for the detailed analysis of immune cell populations. γδ T cells, a subset of T cells, play pivotal roles in immune surveillance and immune aging. Assessing the phenotype and functional capabilities of γδ T cells isolated from whole blood or tissue within the context of human aging yields invaluable insights into the dynamic changes affecting immune function, tissue homeostasis, susceptibility to infections, and inflammatory responses.

Keywords: Cell expansion; Density gradient centrifugation; Flow cytometry; Functional assays; Immunomagnetic separation; Tumor-infiltrating lymphocytes; γδ T cells.

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