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. 2025:2857:223-227.
doi: 10.1007/978-1-0716-4128-6_19.

Antioxidant Features of Humic Products by ABTS Assay

Mariavittoria Verrillo  1   2 , Vincenza Cozzolino  3   4 , Riccardo Spaccini  3   4
Affiliations

Antioxidant Features of Humic Products by ABTS Assay

Mariavittoria Verrillo et al. Methods Mol Biol. 2025.

Abstract

Oxidative stress plays a pivotal role in driving immunosenescence by disrupting cellular homeostasis and impairing immune function. Humic substances exhibit scavenging activity against reactive oxygen species (ROS), inhibit ROS generation via metal chelation, and modulate endogenous antioxidant enzyme activity. Additionally, humic substances display anti-inflammatory effects, further supporting cellular redox balance. Given their antioxidant activity, humic substances hold promise as natural compounds for mitigating oxidative stress-associated immunosenescence. Here we describe the evaluation of antioxidant capacities of humic products by ABTS spectrophotometric assay.

Keywords: Antioxidant properties; Humic products; Natural compounds; ROS balances.

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