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. 2024 Dec;54(12):e2451043.
doi: 10.1002/eji.202451043. Epub 2024 Sep 30.

Liver type 1 innate lymphoid cells undergo apoptosis in murine models of macrophage activation syndrome and are dispensable for disease

Amber De Visscher  1 , Marte Vandeput  1 , Jessica Vandenhaute  1 , Bert Malengier-Devlies  1   2 , Eline Bernaerts  1 , Kourosh Ahmadzadeh  1 , Jessica Filtjens  1 , Tania Mitera  1 , Nele Berghmans  3 , Philippe E Van den Steen  4 , Christin Friedrich  5 , Georg Gasteiger  5 , Carine Wouters  1 , Patrick Matthys  1
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Free article

Liver type 1 innate lymphoid cells undergo apoptosis in murine models of macrophage activation syndrome and are dispensable for disease

Amber De Visscher et al. Eur J Immunol. 2024 Dec.
Free article

Abstract

Macrophage activation syndrome (MAS) exemplifies a severe cytokine storm disorder with liver inflammation. In the liver, classical natural killer (cNK) cells and liver-resident type 1 innate lymphoid cells (ILC1s) dominate the ILC population. Thus far, research has primarily focused on the corresponding role of cNK cells. Considering the liver inflammation and cytokine storm in MAS, liver-resident ILC1s represent an interesting population to explore due to their rapid cytokine production upon environmental triggers. By utilizing a Toll-like receptor (TLR)9- and TLR3:4-triggered MAS model, we showed that ILC1s highly produce IFN-γ and TNF-α. However, activated ILC1s undergo apoptosis and are strongly reduced in numbers, while cNK cells resist inflammation-induced apoptosis. Signs of mitochondrial stress suggest that this ILC1 apoptosis may be driven by inflammation-induced mitochondrial impairment. To study whether early induction of highly cytokine-producing ILC1s influences MAS development, we used Hobit KO mice due to their paucity of liver ILC1s but unaffected cNK cell numbers. Nevertheless, neither the severity of MAS features nor the total inflammatory cytokine levels were affected in these Hobit KO mice, indicating that ILC1s are dispensable for MAS pathogenesis. Collectively, our data demonstrate that ILC1s undergo apoptosis during TLR-triggering and are dispensable for MAS pathogenesis.

Keywords: Apoptosis; Cytokine storm; Hobit knockout mice; Liver type 1 innate lymphoid cells; Macrophage activation syndrome.

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