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Published Erratum
. 2024 Oct 8;121(41):e2418618121.
doi: 10.1073/pnas.2418618121. Epub 2024 Sep 30.

Correction for Cui et al., HKDC1, a target of TFEB, is essential to maintain both mitochondrial and lysosomal homeostasis, preventing cellular senescence

No authors listed
Published Erratum

Correction for Cui et al., HKDC1, a target of TFEB, is essential to maintain both mitochondrial and lysosomal homeostasis, preventing cellular senescence

No authors listed. Proc Natl Acad Sci U S A. .
No abstract available

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Figures

Fig. 5.
Fig. 5.
Complexes of HKDC1 with VDACs regulate mitochondria–lysosome contact and are essential for lysosomal repair. (A) RNA-seq analysis results are shown in Venn diagrams depicting the numbers of DEGs from WT vs. TFEB KO HeLa cells with or without treatment with LLOMe for 1 h followed by washout for 6 h or 12 h. N = 4. (B) Validation of qPCR-detected HKDC1 expression in WT or TFEB KO HeLa cells treated with LLOMe for 1 h followed by washout for 3 h or 10 h. N = 3. (C) Representative Gal3-antibody immunostaining images (Left) and quantification (Right) of damaged lysosomes in HKDC1 knockdown HeLa cells treated with LLOMe for 1 h followed by washout for 3 h or 10 h. Nuclei were stained with DAPI. N = 3. (Scale bar, 10 μm.) (D) Representative IF images (Left) and quantification (Right) of CHMP4B, ALIX, or VPS4 puncta in siLuc- or siHKDC1-transfected HeLa cells treated with 1 mM LLOMe for 0, 30, or 60 min. N = 3. (Scale bar, 10 μm.) (E) VDAC1-, VDAC2-, and VDAC3-3×FLAG were transiently expressed in HeLa cells stably expressing FL HKDC1–mNG. HKDC1-mNG was immunoprecipitated with mNG agarose beads. N = 3. Red arrowheads represent VDAC1, 2, and 3, respectively. (F) Schematic domain structure (Left Upper) of SPLICS reporter to detect lysosome–mitochondria (LY-MT) contact sites. Representative images (Left Lower) and quantification (Right) of SPLICS GFP punctate spots in siLuc- or siHKDC1-treated HeLa cells with or without LLOMe for 2 h. Nuclei were stained with DAPI. N (number of analyzed cells) ≥ 30. (Scale bar, 10 µm.) Values are represented as the mean ± SD, and P-values (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001) were determined by one-way ANOVA with Tukey’s multiple comparison test (B, D, and F) or unpaired t test (C).
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