doi: 10.1111/j.1432-1033.1985.tb09342.x.
Localization of the tubulin binding site for tau protein
- PMID: 3935441
- DOI: 10.1111/j.1432-1033.1985.tb09342.x
Item in Clipboard
Localization of the tubulin binding site for tau protein
Eur J Biochem.
.
Free article
Abstract
Limited proteolysis of tubulin with subtilisin resulted in the removal of the carboxyl-terminal moiety of tubulin subunits. The remaining peptides from both alpha and beta tubulin lacking the carboxyl terminal did not bind to tau factor nor to MAP2 or MAP1. The carboxyl-terminal fragments bind to tau factor and MAP2 and both compete for the same binding sites in the tubulin molecule. Our results suggest that the carboxyl-terminal region of tubulin is a regulatory domain for the assembly of tubulin and the site for interaction with MAPs.
Similar articles
-
Regulatory aspects of the colchicine interactions with tubulin.Mol Cell Biochem. 1987 Jan;73(1):29-36. doi: 10.1007/BF00229373. Mol Cell Biochem. 1987. PMID: 3543651
-
Controlled proteolysis of tubulin by subtilisin: localization of the site for MAP2 interaction.Biochemistry. 1984 Sep 25;23(20):4675-81. doi: 10.1021/bi00315a024. Biochemistry. 1984. PMID: 6388633
-
Common and distinct tubulin binding sites for microtubule-associated proteins.Proc Natl Acad Sci U S A. 1986 Oct;83(19):7162-6. doi: 10.1073/pnas.83.19.7162. Proc Natl Acad Sci U S A. 1986. PMID: 3463956 Free PMC article.
-
Involvement of the carboxyl-terminal domain of tubulin in the regulation of its assembly.Proc Natl Acad Sci U S A. 1984 Oct;81(19):5989-93. doi: 10.1073/pnas.81.19.5989. Proc Natl Acad Sci U S A. 1984. PMID: 6385005 Free PMC article.
-
Effect of estramustine phosphate on the assembly of trypsin-treated microtubules and microtubules reconstituted from purified tubulin with either tau, MAP2, or the tubulin-binding fragment of MAP2.Arch Biochem Biophys. 1987 Aug 15;257(1):123-30. doi: 10.1016/0003-9861(87)90550-9. Arch Biochem Biophys. 1987. PMID: 3115177
Cited by
-
Roles of beta-tubulin residues Ala428 and Thr429 in microtubule formation in vivo.J Biol Chem. 2009 Feb 13;284(7):4283-91. doi: 10.1074/jbc.M807491200. Epub 2008 Dec 13. J Biol Chem. 2009. PMID: 19074767 Free PMC article.
-
Site-directed mutagenesis of alpha-tubulin. Reductive methylation studies of the Lys 394 region.Biophys J. 1993 Mar;64(3):792-802. doi: 10.1016/S0006-3495(93)81440-1. Biophys J. 1993. PMID: 8097117 Free PMC article.
-
New Features about Tau Function and Dysfunction.Biomolecules. 2016 Apr 19;6(2):21. doi: 10.3390/biom6020021. Biomolecules. 2016. PMID: 27104579 Free PMC article. Review.
-
Regulatory aspects of the colchicine interactions with tubulin.Mol Cell Biochem. 1987 Jan;73(1):29-36. doi: 10.1007/BF00229373. Mol Cell Biochem. 1987. PMID: 3543651
-
Expression of specific tubulin isotypes increases during regeneration of injured CNS neurons, but not after the application of brain-derived neurotrophic factor (BDNF).J Neurosci. 1997 Jun 15;17(12):4623-32. doi: 10.1523/JNEUROSCI.17-12-04623.1997. J Neurosci. 1997. PMID: 9169523 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
