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. 1979 Nov 1;52(1):143-7.
doi: 10.1007/BF00284609.

A highly sensitive peroxidase-antiperoxidase method for detection of H-Y antigen on cultivated human fibroblasts

A highly sensitive peroxidase-antiperoxidase method for detection of H-Y antigen on cultivated human fibroblasts

U Müller et al. Hum Genet. .

Abstract

A peroxidase-anti-perioxidase method for the detection of H-Y antigen at the single cell level is described. The efficiency of the test was examined in cultivated fibroblasts derived from control subjects and from XX males and a true hermaphrodite. For comparison, H-Y antigen was determined in blood cells of the same probands using the cytotoxic test. The finding of H-Y positive fibroblasts in the intersex patients has implications for the origin of these disorders.

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