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Review
. 2024 Oct 4;108(1):480.
doi: 10.1007/s00253-024-13315-y.

Improvement strategies for transient gene expression in mammalian cells

Yushun Fu  1   2 , Zimeng Han  1   2 , Wanting Cheng  2 , Shuaichen Niu  1 , Tianyun Wang  3   4 , Xiaoyin Wang  5   6
Affiliations
Review

Improvement strategies for transient gene expression in mammalian cells

Yushun Fu et al. Appl Microbiol Biotechnol. .

Abstract

Mammalian cells are suitable hosts for producing recombinant therapeutic proteins, with Chinese hamster ovary (CHO) and human embryonic kidney 293 (HEK293) cells being the most commonly used cell lines. Mammalian cell expression system includes stable and transient gene expression (TGE) system, with the TGE system having the advantages of short cycles and simple operation. By optimizing the TGE system, the expression of recombinant proteins has been significantly improved. Here, the TGE system and the detailed and up-to-date improvement strategies of mammalian cells, including cell line, expression vector, culture media, culture processes, transfection conditions, and co-expression of helper genes, are reviewed. KEY POINTS: • Detailed improvement strategies of transient gene expression system of mammalian cells are reviewed • The composition of transient expression system of mammalian cell are summarized • Proposed optimization prospects for transient gene expression systems.

Keywords: CHO cell; HEK293 cell; Optimization; Recombinant protein; Transient gene expression.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
Schematic workflow of transient expression process in mammalian cells
Fig. 2
Fig. 2
Strategies to improve transient expression in mammalian cells
See this image and copyright information in PMC

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