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Review
. 2024 Dec:89:102934.
doi: 10.1016/j.sbi.2024.102934. Epub 2024 Oct 3.

Recent advances in correlative cryo-light and electron microscopy

Joshua A Pierson  1 , Jie E Yang  2 , Elizabeth R Wright  3
Affiliations
Review

Recent advances in correlative cryo-light and electron microscopy

Joshua A Pierson et al. Curr Opin Struct Biol. 2024 Dec.

Abstract

Correlative light and electron microscopy (CLEM) pipelines serve to integrate the imaging modalities of fluorescence light microscopy (FLM) and cryogenic electron microscopy (cryo-EM) to produce contextually relevant high-resolution structural snapshots of biological systems. Innovations in sample preparation, instrumentation, imaging, and data processing have advanced the field of cryo-EM. This review focuses on prior work and recent developments in the field of cryo- EM that support further integration of technologies for correlative microscopy workflows.

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Conflict of interest statement

Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

References

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