Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Oct 3;84(19):3576-3577.
doi: 10.1016/j.molcel.2024.09.014.

50 years in the making: acp3U, an amino-acid-containing nucleoside, links N-glycans and RNA in glycoRNA

Kfir B Steinbuch  1 , Yitzhak Tor  2
Affiliations

50 years in the making: acp3U, an amino-acid-containing nucleoside, links N-glycans and RNA in glycoRNA

Kfir B Steinbuch et al. Mol Cell. .

Abstract

In a recent publication in Cell, Xie et al.1 report a sensitive and scalable method for the detection and characterization of native glycoRNAs and identify acp3U, an abundant modified nucleoside discovered 50 years ago in tRNAPhe, as one of the primary attachment sites for N-glycans.

PubMed Disclaimer

Conflict of interest statement

Declaration of interests The authors declare no competing interests.

Figures

Figure 1.
Figure 1.. Deciphering the molecular structure of glycoRNA
Cartoon representation of a generic sialicglycoRNA (left). An amino-acid-containing nucleoside, acp3U, a verified N-glycan anchoring nucleoside, is shown next to other carboxylic-acid- and carboxamide-containing nucleosides, which may also serve as attachment sites for oligosaccharides (right).
See this image and copyright information in PMC

Comment on

Similar articles

See all similar articles

References

    1. Xie Y, Chai P, Till NA, Hemberger H, Lebedenko CG, Porat J, Watkins CP, Caldwell RM, George BM, Perr J, et al. (2024). The modified RNA base acp3U is an attachment site for N-glycans in glycoRNA. Cell 187, 5228–5237.e7. 10.1016/j.cell.2024.07.044. - DOI - PMC - PubMed
    1. Flynn RA, Pedram K, Malaker SA, Batista PJ, Smith BAH, Johnson AG, George BM, Majzoub K, Villalta PW, Carette JE, and Bertozzi CR (2021). Small RNAs are modified with N-glycans and displayed on the surface of living cells. Cell 184, 3109–3124.e22. 10.1016/j.cell.2021.04.023. - DOI - PMC - PubMed
    1. Kasai H, Nakanishi K, Macfarlane RD, Torgerson DF, Ohashi Z, McCloskey JA, Gross HJ, and Nishimura S (1976). Letter: The structure of Q* nucleoside isolated from rabbit liver transfer ribonucleic acid. J. Am. Chem. Soc 98, 5044–5046. 10.1021/ja00432a071. - DOI - PubMed
    1. Zhang H, Li XJ, Martin DB, and Aebersold R (2003). Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry. Nat. Biotechnol 21, 660–666. 10.1038/nbt827. - DOI - PubMed
    1. . Xie Y, De Luna Vitorino FN, Chen Y, Lempiäinen JK, Zhao C, Steinbock RT, Lin Z, Liu X, Zahn E, Garcia AL, et al. (2023). SWAMNA: a comprehensive platform for analysis of nucleic acid modifications. Chem. Commun 59, 12499–12502. 10.1039/d3cc04402e. - DOI - PMC - PubMed