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. 1985:(16):287-90.

Synthesis and expression of the native RNase T1 gene and several mutant genes

S Nishikawa, H Morioka, T Tokunaga, Y Aoyama, S Kikyotani, K Fujimoto, K Yanase, T Tanaka, S Uesugi, E Ohtsuka, et al.
  • PMID: 3937139

Synthesis and expression of the native RNase T1 gene and several mutant genes

S Nishikawa et al. Nucleic Acids Symp Ser. 1985.

Abstract

RNase T1 gene and several mutant genes were constructed by joining of chemically synthesized deoxyoligonucleotides. These genes were inserted into an expression vector and expressed as fused protein in E. coli. RNase T1 and its mutant enzymes were liberated by cyanogen bromide treatment and their activities were measured.

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