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. 2024 Sep 30;21(3):e20240059.
doi: 10.1590/1984-3143-AR2024-0059. eCollection 2024.

Approaches to improve in vitro survival, growth, and maturation of caprine oocytes: main results from LAMOFOPA-Brazil

José Ricardo de Figueiredo  1 , Ana Flávia Bezerra da Silva  1 , Laritza Ferreira de Lima  1
Affiliations

Approaches to improve in vitro survival, growth, and maturation of caprine oocytes: main results from LAMOFOPA-Brazil

José Ricardo de Figueiredo et al. Anim Reprod. .

Abstract

This brief review delves into the topic of in vitro follicle culture for in vitro embryo production, with a particular emphasis on goat models. Specifically, we examine the main findings from LAMOFOPA-Brazil over the last 20 years, highlighting the challenges posed by oxidative stress and epigenetic changes. Our focus is on strategies to improve follicular development and oocyte maturation. Furthermore, we underscore the valuable role of the antioxidant anethole in optimizing the efficacy of in vitro follicle culture and improving outcomes in in vitro embryo production.

Keywords: anethole; caprine; in vitro follicle culture; oocyte growth.

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Conflict of interest statement

Conflicts of interest: The authors have no conflict of interest to declare.

Figures

Figure 1
Figure 1. Approaches to improve in vitro survival, growth, and maturation of caprine oocytes. 1- Activation (recruitment) of primordial follicles; 2- Production of early secondary follicles from primordial follicles; 3- Transition from late preantral to early antral follicles; 4- Progression from early to late antral follicles; and 5- Impact of anethole during follicular (from primordial to late antral follicles) and oocyte in vitro maturation (IVM) for in vitro embryo production (IVEP). FSH: Follicle-stimulating hormone; GH: Growth hormone; and LH: Luteinizing hormone. ↑: High (10 µg/mL) and ↓: Low (10 ng/mL) insulin.
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