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. 2024 Oct 7;9(10):1355-1359.
doi: 10.1080/23802359.2024.2412230. eCollection 2024.

The complete mitogenome and phylogenetic analysis of Thalassa montezumae Mulsant, 1850 (Coleoptera: coccinellidae)

Immacolata Iovinella  1 , Lucrezia Giovannini  1 , Giuseppe Mazza  1 , Bryan Naqqi Manco  2 , Agostino Strangi  1
Affiliations

The complete mitogenome and phylogenetic analysis of Thalassa montezumae Mulsant, 1850 (Coleoptera: coccinellidae)

Immacolata Iovinella et al. Mitochondrial DNA B Resour. .

Abstract

Thalassa montezumae Mulsant is a coccinellid species recently discovered as predator of the pine tortoise scale Toumeyella parvicornis. In this study, the complete mitochondrial genome of T. montezumae collected in Turks and Caicos Islands in 2023 was sequenced using next-generation sequencing techniques. The circular mitochondrial genome is 16,981 bp long and contains 13 protein coding genes, 22 transfer RNA, and 2 rRNA genes. Gene order is identical to that of other Coccinellidae. Phylogenetic analysis confirms structure of Coccinellidae families and tribes.

Keywords: Toumeyella natural enemies; biological control; complete mitochondrial genome; lady beetle; scale insect predator.

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Conflict of interest statement

No potential conflict of interest was reported by the authors.

Figures

Figure 1.
Figure 1.
Thalassa montezumae male (on the left) and female (on the right). Picture taken with SMZ-25 stereomicroscope (NIKON) by Luca Madonni.
Figure 2.
Figure 2.
Gene arrangement in mitochondrial genome of Thalassa montezumae (Accession: PP865227). Visualization of MITOS2 and tRNAscan-SE 2.0 prediction using Geneious v. 2023.2.1.
Figure 3.
Figure 3.
Codon usage in mitochondrial coding sequences of Thalassa montezumae.
Figure 4.
Figure 4.
Phylogenetic tree of coccinellid species computed from 13 concatenated mitochondrial PCGs. Near the branches in black the neighbor joining jackknife values and in blue the maximum likelihood bootstrap values. The following sequences were used: NC042417 (Seo et al. 2019), NC067078 (NCBI Direct Submission), NC058236 (NCBI Direct Submission), MW845811 (unpublished), MZ334467 (Cui et al. 2021), NC046481 (Hao et al. 2019), NC064320 (NCBI Direct Submission), NC036272 (NCBI Direct Submission), NC061950 (Zhu et al. 2022), NC066052 (NCBI Direct Submission), NC066406 (Yan et al. 2020), KT780638 (NCBI Direct Submission), NC050855 (Magro et al. 2020), MN164642 – MN164648 (Magro et al. 2020), PP865227 (this work), NC064321 (Zhang et al. 2023), NC023469 (Behere et al. 2016), NC041172 (NCBI Direct Submission), MN447521 (Nattier and Salazar 2019), NC008221 (Fang et al. 2016).
See this image and copyright information in PMC

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