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. 2024 Sep 6:2024:10.17912/micropub.biology.001253.
doi: 10.17912/micropub.biology.001253. eCollection 2024.

Massive bowel resection modulates the expression of genes involved in lipid and cholesterol metabolism in rats

Taku Hebiguchi  1   2 , Mayako Morii  1 , Ryo Watanabe  1 , Hiroaki Yoshino  1   3 , Yoshihiro Mezaki  4
Affiliations

Massive bowel resection modulates the expression of genes involved in lipid and cholesterol metabolism in rats

Taku Hebiguchi et al. MicroPubl Biol. .

Abstract

We have previously shown that vitamin A-absorptive function was enhanced in bowel-resected rats via increased expression of cellular retinol-binding protein II (CRBP II). Recently, CRBP II was shown to bind not only to retinol but also to monoacylglycerols to modulate gut endocrine signaling. We hypothesized that the increased CRBP II in bowel-resected rats had broader effects than vitamin A metabolism. Acetyl-CoA carboxylase 1 (fatty-acid biosynthesis) and sterol O-acyltransferase 1 (cholesterol esterification) expressions were down-regulated in the bowel-resected rats. Adjustment of nutritional absorption may take place in a limited area of the small intestine by the modulation of gene expression.

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Conflict of interest statement

The authors declare that there are no conflicts of interest present.

Figures

Figure 1. Quantitative analysis of mRNA levels in short bowel (SB) and sham rats
Figure 1. Quantitative analysis of mRNA levels in short bowel (SB) and sham rats
The mRNA levels of sterol regulatory element-binding transcription factor 1 ( Srebf1 ), acetyl-CoA carboxylase-alpha ( Acaca ), acyl-CoA oxidase 1 ( Acox1 ), sterol O-acyltransferase 1 ( Soat1 ), lecithin:cholesterol acyltransferase ( Lcat ), and cholesterol 7-alpha-hydroxylase 1 ( Cyp7a1 ) were assessed by quantitative RT-PCR. Data are expressed as the means ± SD. *P < 0.05 was considered to indicate a statistically significant difference.
See this image and copyright information in PMC

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