Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Oct;14(10):e70047.
doi: 10.1002/ctm2.70047.

Bioactive compounds from ShenFuShanYuRou decoction enhance Treg cell function against hemorrhagic shock injury via Stat1- and Gbp5-dependent FOXP3 induction

Qingxia Huang  1   2 , Mingxia Wu  2 , Lu Ding  1   2 , Chen Guo  2 , Yisa Wang  2 , Zhuo Man  3 , Hang Su  2 , Jing Li  2 , Jinjin Chen  2 , Yao Yao  2 , Zeyu Wang  2 , Daqing Zhao  2 , Linhua Zhao  4 , Xiaolin Tong  4 , Xiangyan Li  2
Affiliations

Bioactive compounds from ShenFuShanYuRou decoction enhance Treg cell function against hemorrhagic shock injury via Stat1- and Gbp5-dependent FOXP3 induction

Qingxia Huang et al. Clin Transl Med. 2024 Oct.
No abstract available

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

FIGURE 1
FIGURE 1
SFSY treatment improves the Treg cell function in vivo and in vitro. (A) qPCR assay was used to analyze the transcripts of Tbx21. (B) The Treg cell percentages in peripheral blood mononuclear cells (PBMCs) and lung cells were analyzed by flow cytometry. (C) The co‐localization of FOXP3 and DAPI was analyzed by immunofluorescence and confocal microscopy in the lungs. (D) The Treg cell function was analyzed by immunofluorescence staining and co‐localization analysis in Treg cells.
FIGURE 2
FIGURE 2
The potential molecular mechanisms underlying SFSY‐mediated Treg cell function were analyzed by transcriptomic analysis. (A, B) The MAP and heart rate were continuously monitored by a digital biological signal acquisition and processing system. (C) The blood gas and electrolytes were analyzed after the arterial blood was collected into anticoagulant tubes with heparin lithium. (D) After resuscitation, the arterial blood was collected from the aorta, and an anticoagulant with EDTA‐2K was added. The complete blood count was detected by a haematology analyzer. (E) The ALT (alanine aminotransferase), AST (aspartate aminotransferase), and CK (creatine kinase) concentrations in serum were analyzed by biochemical method. (F) The microcirculation in the intestine had been measured and analyzed by a laser speckle imaging system before the rats were sacrificed. (G) The principal component analysis showed significant differences in mRNA expression among the Sham, HS/R, and HS/R + SFSY groups. (H) The volcano plot shows the transcriptional changes identified in the HS/R group vs. the HS/R + SFSY group. (I) The heat map displays the differentially expressed genes of the immunity‐related pathways in these three groups. Red and blue represent high and low levels of expression of the indicated genes, respectively. (J) The association between differentially expressed genes and FOXP3 expression in the lungs was analyzed by correlation coefficient plots.
FIGURE 3
FIGURE 3
Stat1 inhibition abrogates the improved efficacy of Treg function by ginsenoside Ro or hypaconitine treatment in Treg cell polarizing conditions. (A) Immunoblot analysis of p‐Stat1, Stat1, and FOXP3 in Treg cells after ginsenoside Ro, hypaconitine, or/and fludarabine treatment under Treg cell polarizing conditions. (B, C) Immunofluorescence was used to analyze the co‐localization of FOXP3 and DAPI in Treg cells. (D) The level of CXCL10 mRNA was analyzed by qPCR assay. (E, F) The mRNA expression of mitochondrial function was analyzed by qPCR in Treg cells. (G) The Treg cells were pretreated with ginsenoside Ro or/and Fludarabine for 3 days, and the mitochondrial counts and mitochondrial fission were analyzed by living‐cell staining with MitoTracker, Lyso‐Tracker, and Hoechst 33258 probes. The expression of TOM20 was analyzed by immunofluorescence. (H) The mitochondrial function was recorded by continuous injections with inhibitors, including oligomycin, carbonyl cyanide 4‐trifluoromethoxy‐phenylhydrazone, and a mixture of antimycin A and rotenone.
FIGURE 4
FIGURE 4
Loganic acid inhibits Gbp5‐mediated pyroptosis to enhance Treg cell function. (A) Immunoblot analysis of Gbp5, GSDMD, and FOXP3 in Treg cells after loganic acid (LA) or/and LPS incubation under Treg cell polarizing conditions. (B) Treg cells were infected with NC or sh‐Gbp5 lentivirus, and the infection efficiency was analyzed by western blot. (C) The effect of LA on decreasing Gbp5‐mediated pyroptosis was analyzed by western blot. (D) The co‐localization of FOXP3 and DAPI in Treg cells was analyzed by immunofluorescence staining. (E, F) The pyroptosis and apoptosis of the Treg cells were evaluated using calcein/propidium iodide (PI) staining and Annexin V/PI staining kits.
See this image and copyright information in PMC

References

    1. Cannon J. Hemorrhagic shock. New Eng J Med 2018;378:370‐379. doi:10.1056/NEJMra1705649 - DOI - PubMed
    1. Manson J, Hoffman R, Chen S, et al. Innate‐like lymphocytes are immediate participants in the hyper‐acute immune response to trauma and hemorrhagic shock. Front Immunol. 2019;10:1501. doi:10.3389/fimmu.2019.01501 - DOI - PMC - PubMed
    1. Vasanthakumar A, Chisanga D, Blume J, et al. Sex‐specific adipose tissue imprinting of regulatory T cells. Nature. 2020;579:581‐585. doi:10.1038/s41586-020-2040-3 - DOI - PMC - PubMed
    1. Liu X, Zhang W, Han Y, et al. FOXP3 regulatory T cell perturbation mediated by the IFNγ‐STAT1‐IFITM3 feedback loop is essential for anti‐tumor immunity. Nat Commun. 2024;15:122. doi:10.1038/s41467-023-44391-9 - DOI - PMC - PubMed
    1. Meier J, Larner A. Toward a new STATe: the role of STATs in mitochondrial function. Semin Immunol. 2014;26:20‐28. doi:10.1016/j.smim.2013.12.005 - DOI - PMC - PubMed

MeSH terms

LinkOut - more resources