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. 2024 Nov;53(6):e13112.
doi: 10.1111/ahe.13112.

PRP Influences Maturation and Fertilisation of Immature Mouse Oocytes

Sanaz Alaee  1   2   3 , Fatemeh Zal  3   4 , Vahid Razban  2   5 , Tahereh Talaei-Khozani  6   7 , Saeed Shokri  8 , Zahra Khodabandeh  2
Affiliations

PRP Influences Maturation and Fertilisation of Immature Mouse Oocytes

Sanaz Alaee et al. Anat Histol Embryol. 2024 Nov.

Abstract

In vitro maturation (IVM) of immature oocytes is a valuable method to enhance the rate of mature oocytes available for fertilisation. In the current study, platelet-rich plasma (PRP) was employed in IVM medium of immature oocytes. Harvested germinal vesicle stage oocytes with cumulus cells from female mature BALB/c mice divided into two groups of control and experiment. In the experimental group, GV oocytes matured in the IVM medium supplemented with 5% PRP, while in the control group, GV oocytes matured in the IVM medium without PRP. The percentage of GV, MI, MII and degenerated oocytes, zona pellucida thickness, perivitelline space size, diameter of mature oocytes, gene expression of apoptosis-related factors and subsequent development of matured oocytes were assessed. The PRP group displayed significantly improved outcomes in various parameters, including a higher proportion of MII and fertilised oocytes, cleavage and blastocyst embryos, compared to the control group. Moreover, the thickness of the zona pellucida was significantly lower in the PRP group than in the control group (p < 0.05). Furthermore, the PRP group demonstrated a significant decrease in the expression of transcripts associated with apoptosis (Bax and caspase-3); however, in the PRP group, a substantial increase in the expression of Bcl2l1, an apoptosis inhibitor, was observed when compared to the control group (p < 0.05). In conclusion, addition of PRP to the IVM culture media significantly increased oocyte maturation rate, leading to improved fertilisation and subsequent embryonic development. This enhancement highlights the positive influence of PRP on overall in vitro maturation efficiency and early embryonic stages.

Keywords: apoptosis; embryo development; in vitro oocyte maturation; platelet‐rich plasma.

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References

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