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. 2024 Oct:59 Suppl 3:e14600.
doi: 10.1111/rda.14600.

The Cryotop vitrification system is competent for the simultaneous cryopreservation of large numbers of pig in vitro-produced blastocysts

A González-Plaza  1   2 , M Garcia-Canovas  1   2 , I Parrilla  1   2 , H Rodriguez-Martinez  3 , M A Gil  1   2 , E A Martinez  1   2 , C Cuello  1   2
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Free article

The Cryotop vitrification system is competent for the simultaneous cryopreservation of large numbers of pig in vitro-produced blastocysts

A González-Plaza et al. Reprod Domest Anim. 2024 Oct.
Free article

Abstract

This study aimed to compare the effectiveness, in terms of viability and quality post-warming, when vitrifying in vitro-produced (IVP) pig blastocysts with either a modified Cryotop (n = 161; 20 blastocysts/device) or the conventional Superfine Open Pulled Straw (SOPS; n=160; 5-6 blastocysts/device systems. Blastocyst viability, morphology, and apoptosis parameters were evaluated after 24 h of in vitro culture. The Cryotop system yields better results in terms of higher embryo viability and total cell numbers (p < .05) and lower apoptosis (p < .05) parameters than the SOPS procedure, defining a high effectiveness to simultaneously vitrify 20 pig IVP blastocysts at one time, thus increasing the yield of IVP blastocysts readily available for embryo transfer.

Keywords: Cryotop; SOPS; in vitro‐produced blastocysts; porcine; vitrification.

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References

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