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. 1986 Jan 1;19(1-2):57-66.
doi: 10.1016/0165-4608(86)90372-9.

Methodologic advances in the cytogenetic analysis of human solid tumors

J Trent, K Crickard, Z Gibas, A Goodacre, S Pathak, A A Sandberg, F Thompson, J Whang-Peng, S Wolman

Methodologic advances in the cytogenetic analysis of human solid tumors

J Trent et al. Cancer Genet Cytogenet. .

Abstract

The major obstacle to successful cytogenetic analysis of human solid tumors is the acquisition of sufficient numbers of good quality metaphases for detailed cytogenetic analysis. At present, no single methodologic approach has been proven to provide successful chromosomal analysis of all human solid tumors. The technical aspects of cell culture, chromosome harvesting, and chromosome banding were the focus of considerable discussion during the First Workshop on Chromosomes in Solid Tumors. This report provides summaries of several technical protocols, emanating from several different laboratories, which have contributed to successful chromosome analysis of a variety of human solid tumors.

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