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. 2024 Oct 15;24(1):965.
doi: 10.1186/s12870-024-05668-3.

Effects of alkaline salt stress on growth, physiological properties and medicinal components of clonal Glechoma longituba (Nakai) Kupr

Donghai Wang #  1 , Fangshuai Song #  1 , Yitong Zhou  2 , Tingting Zhong  2 , Yuyan Zhang  2 , Qiao Deng  2 , Xinqi Wang  2 , Siqi Wang  2 , Daocai Wang  1   2 , Xiqiang Zhu  1   2 , Ning Jiang  1   2 , Xiaopeng Liu  3   4
Affiliations

Effects of alkaline salt stress on growth, physiological properties and medicinal components of clonal Glechoma longituba (Nakai) Kupr

Donghai Wang et al. BMC Plant Biol. .

Abstract

Background: Glechoma longituba, recognized as a medicinal plant, provides valuable pharmaceutical raw materials for treating various diseases. Saline-alkali stress may effectively enhance the medicinal quality of G. longituba by promoting the synthesis of secondary metabolites. To investigate the changes in the primary medicinal components of G. longituba under saline-alkali stress and improve the quality of medicinal materials, Na2CO3 was applied to induce short-term stress under different conditions and the biomass, physiologically active substances and primary medicinal components of G. longituba were measured in this study.

Results: Under alkaline salt stress, the activities of catalase (CAT), superoxide dismutase (SOD), peroxidase (POD), and ascorbate peroxidase (APX) were elevated in G. longituba, accompanied by increased accumulation of proline (Pro) and malondialdehyde (MDA). Furthermore, analysis of the medicinal constituents revealed that G. longituba produced the highest levels of soluble sugars, flavonoids, ursolic acid, and oleanolic acid under 0.6% Na2CO3 stress for 48 h, 0.2% Na2CO3 stress for 72 h, 0.4% Na2CO3 stress for 12 h, and 0.4% Na2CO3 stress for 8 h, respectively.

Conclusions: Short-term Na2CO3 stress enhances the synthesis of medicinal components in G. longituba. By manipulating stress conditions, the production of various medicinal substances could be optimized. This approach may serve as a basis for the targeted cultivation of G. longituba, offering potential applications in the treatment of diverse diseases.

Keywords: Glechoma longituba; Active substance; Antioxidant; Medicinal constituents; Salt stress.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
Effects of concentrations and durations of Na2CO3stress on the growth status of G. longituba
Fig. 2
Fig. 2
Effects of concentrations and durations of Na2CO3 stress on H2O2 content in G. longituba (mean ±formula image SE)
Fig. 3
Fig. 3
Effects of concentrations and durations of Na2CO3 stress on MDA content in G. longituba (mean ±formula image SE)
Fig. 4
Fig. 4
Effects of concentrations and durations of Na2CO3 stress on antioxidant enzyme activities in G. longituba (mean ±formula image SE)
Fig. 5
Fig. 5
Effects of concentrations and durations of Na2CO3 stress on accumulation of Pro and soluble sugar in G. longituba (mean ±formula image SE)
Fig. 6
Fig. 6
Effects of concentrations and durations of Na2CO3 stress on total flavonoids content in G. longituba (mean ±formula image SE)
Fig. 7
Fig. 7
Effects of concentrations and durations of Na2CO3 stress on ursolic acid and oleanolic acid content in G. longituba (mean ±formula image SE)
Fig. 8
Fig. 8
Correlation analysis of physiological and biochemical properties of G. longituba under the stress of 0.2% (a), 0.4% (b), 0.6% (c), and 0.8% (d) alkali salt concentrations, respectively. * indicates significance at a 5% level, ** shows significance at a 1% level, and color depth denotes correlation coefficient. The strength of the correlation is indicated by the size of the circle
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