Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2024 Dec;13(32):e2402876.
doi: 10.1002/adhm.202402876. Epub 2024 Oct 14.

Controlling Cell Interactions with DNA Directed Assembly

Katelyn Mathis  1   2 , Clement T Y Chan  1   2 , Brian Meckes  1   2
Affiliations
Review

Controlling Cell Interactions with DNA Directed Assembly

Katelyn Mathis et al. Adv Healthc Mater. 2024 Dec.

Abstract

The creation of complex cellular environments is critical to mimicking tissue environments that will play a critical role in next-generation tissue engineering, stem cell programming, and therapeutic screening. To address this growing need, techniques capable of manipulating cell-cell and cell-material interactions are required that span single-cell to 3D tissue architectures. DNA programmed assembly and placement of cells present a powerful technique for the bottom-up synthesis of living microtissues for probing key questions in cell-cell and cell-material-driven behaviors through its refined control over placement and architecture. This review examines the current state of the art in the programming of cellular interactions with DNA and its applications spanning tissue model building, fundamental cellular biology, and cell manipulation for measurements across a host of applications.

Keywords: 3D printing; DNA programming; cell assembly; lithography; organoids.

PubMed Disclaimer

Conflict of interest statement

Conflict of Interest

The authors declare no conflict of interest.

Similar articles

See all similar articles

References

    1. Fontoura JC; Viezzer C; dos Santos FG; Ligabue RA; Weinlich R; Puga RD; Antonow D; Severino P; Bonorino C Comparison of 2D and 3D cell culture models for cell growth, gene expression and drug resistance. Materials Science & Engineering C 2020, 107, 110264. - PubMed
    1. Stelzer EHK; Pampaloni F; Reynaud EG The third dimension bridges the gap between cell culture and live tissue. Nature Reviews Molecular Cell Biology 2007, 8, 839–845. - PubMed
    1. Sharkey KA; Mawe GM Neuroimmune and epithelial interactions in intestinal inflammation. 2002, 2, 669–677. - PubMed
    1. Hormuzdi SG; Filippov MA; Mitropoulou G; Monyer H; Bruzzone R Electrical synapses: a dynamic signaling system that shapes the activity of neuronal networks. 2004, 1662, 113–137. - PubMed
    1. Berridge MV; Herst PM; Rowe MR; Schneider R; McConnell MJ Mitochondrial transfer between cells: Methodological constraints in cell culture and animal models. Analytical biochemistry 2018, 552, 75–80. - PubMed

LinkOut - more resources